dc.contributor.author | Steeves, Helena | |
dc.date.accessioned | 2021-01-07T18:37:55Z | |
dc.date.available | 2021-01-07T18:37:55Z | |
dc.date.issued | 2021-01-07T18:37:55Z | |
dc.identifier.uri | http://hdl.handle.net/10222/80181 | |
dc.description.abstract | The goal of this research was to develop a fermentative chitin extraction method from Atlantic lobster shell waste. Results showed initial inoculum ratio was the most statistically significant variable in chitin extraction efficiency at a 5% shell ratio. Novel pre-treatments (MAE and UAE) were not found to have a significant influence on extraction efficiency under the tested conditions regardless of the presence of lactose in the pre-treated slurry. As determined by degree of decalcification and deproteinization as well as further measurement of fermentation dynamics, the most efficient of the tested formulations was 5% shell: 5% lactose: 5% inoculum (w/v) without a pre-treatment. The fermentation achieved 95.0% decalcification and 72.7% deproteinization when maintained at 37°C for 5 days with constant stirring. This fermentative approach using lactose and lactobacilli inoculum successfully yielded high levels of mineral and protein removal however further studies are required to optimize the process. | en_US |
dc.language.iso | en | en_US |
dc.subject | Chitin | en_US |
dc.subject | Fermentation | en_US |
dc.subject | Lactic Acid Bacteria | en_US |
dc.subject | Lobster shells | en_US |
dc.title | Developing a Sustainable Bioprocess for Chitin Extraction from Atlantic Lobster Shell Waste | en_US |
dc.type | Thesis | en_US |
dc.date.defence | 2019-11-14 | |
dc.contributor.department | Department of Process Engineering and Applied Science | en_US |
dc.contributor.degree | Master of Applied Science | en_US |
dc.contributor.external-examiner | Dr. Junzeng Zhang | en_US |
dc.contributor.graduate-coordinator | Dr. Suzanne Budge | en_US |
dc.contributor.thesis-reader | Dr. Amyl Ghanem | en_US |
dc.contributor.thesis-supervisor | Dr. Beth Mason | en_US |
dc.contributor.thesis-supervisor | Dr. Su-Ling Brooks | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |