dc.contributor.author | Singh, Gillian Kristina | |
dc.date.accessioned | 2019-08-26T14:41:49Z | |
dc.date.available | 2019-08-26T14:41:49Z | |
dc.date.issued | 2019-08-26T14:41:49Z | |
dc.identifier.uri | http://hdl.handle.net/10222/76304 | |
dc.description.abstract | Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of the inflammatory endothelial cell (EC) cancer, Kaposi’s Sarcoma (KS). Many inflammatory mRNAs (ARE-mRNAs) are subject to constitutive decay in cytoplasmic ribonucleoprotein granules called processing bodies (PB). The viral protein KapB contributes to this inflammatory phenotype by inducing PB disassembly and a correlative increase in ARE-mRNA stabilization. However, the precise molecular mechanism governing KapB-mediated PB disassembly is unclear. My research uncovered a novel link between selective autophagy (SA) and PB disassembly. During SA, molecular-adapter proteins, such as NDP52, selectively bind and target cargo for degradation. I found that KapB-mediated PB disassembly and stabilization of a reporter ARE-mRNA requires NDP52. Moreover, overexpression of authentic NDP52, but not mutated NDP52, rescued KapB-mediated PB disassembly in the context of NDP52 knockdown. Collectively, these data suggest KapB hijacks SA machinery to disassemble PBs, thus contributing to the proinflammatory environment beneficial for tumourigenesis. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Autophagy | en_US |
dc.subject | Selective Autophagy | en_US |
dc.subject | KSHV | en_US |
dc.subject | Processing Bodies | en_US |
dc.subject | Kaposin B | en_US |
dc.subject | ARE-mRNAs | en_US |
dc.title | KAPOSI’S SARCOMA-ASSOCIATED HERPESVIRUS PROTEIN, KAPOSIN B INDUCES THE SELECTIVE CATABOLISM OF PROCESSING BODIES | en_US |
dc.date.defence | 2019-08-07 | |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Francesca Di Cara | en_US |
dc.contributor.graduate-coordinator | Dr. Zhenyu Cheng | en_US |
dc.contributor.thesis-reader | Dr. Thomas Pulinilkunnil | en_US |
dc.contributor.thesis-supervisor | Dr. Jennifer Corcoran | en_US |
dc.contributor.thesis-supervisor | Dr. Craig McCormick | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |