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dc.contributor.authorMerovitch, Neil
dc.date.accessioned2016-08-26T15:13:15Z
dc.date.available2016-08-26T15:13:15Z
dc.identifier.urihttp://hdl.handle.net/10222/72114
dc.description.abstractZebrafish are emerging as a novel model for studying learning and memory. However, the number of behavioural paradigms which minimize handling stress and are suited to their social nature is limited. We developed an automated learning paradigm to condition groups of adult and juvenile zebrafish in their home tanks. Fish consistently learned to associate an auditory stimulus with the presentation of food and showed robust conditioned responses as early as the 5th trial. Memory of the association persisted for at least 2 days after training, when fish were tested either as groups or as individuals. This retention in juveniles was associated with increased immunoreactivity to phosphorylated ERK, a marker of neural activity, in the dorsolateral telencephalon. This simple paradigm permits scalable conditioning of zebrafish with minimal intervention, reducing variability and labour-intensiveness. In addition, these results support the use of phosphorylated ERK to examine the neural correlates of learning and memory.en_US
dc.language.isoenen_US
dc.subjectLearningen_US
dc.subjectMemoryen_US
dc.subjectZebrafishen_US
dc.subjectNeural activityen_US
dc.subjectBehaviouren_US
dc.subjectHigh-throughputen_US
dc.subjectNeuroscienceen_US
dc.subjectZebra danio
dc.subjectZebra danio - Behavior
dc.titleA simple automated system for appetitive conditioning of zebrafish in their home tanks and studying underlying neural activationen_US
dc.typeThesisen_US
dc.date.defence2016-08-22
dc.contributor.departmentDepartment of Physiology & Biophysicsen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Steven Barnesen_US
dc.contributor.graduate-coordinatorDr. Valerie Chappeen_US
dc.contributor.thesis-readerDr. Paivi Torkkelien_US
dc.contributor.thesis-readerDr. Simon Gadboisen_US
dc.contributor.thesis-supervisorDr. Roger Crollen_US
dc.contributor.thesis-supervisorDr. Alan Fineen_US
dc.contributor.ethics-approvalReceiveden_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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