dc.contributor.author | Tanner, Kaitlyn | |
dc.date.accessioned | 2016-01-14T18:03:21Z | |
dc.date.available | 2016-01-14T18:03:21Z | |
dc.date.issued | 2016-01-14T18:03:21Z | |
dc.identifier.uri | http://hdl.handle.net/10222/65292 | |
dc.description.abstract | Shigella spp. cause severe diarrheal disease known as shigellosis and are a major
problem is countries where clean water and sanitation are lacking. Shigella spp. use a
Type 3 Secretion System to deliver effector proteins into the cytosol of infected human
cells. My thesis focuses on an effector of Shigella flexneri, IpaH9.8, and its role in
pathogenesis. IpaH9.8 is a member of a structurally related family of enzymes called the
novel E3 ligases (NELs). NEL-domain proteins are E3 ubiquitin ligases that target host
proteins to alter their fate. I show that IpaH9.8 ubiquitinates ZKSCAN3, a negative
regulator of genes required for autophagy, in a cell free system and induces autophagy in
vitro. My results are consistent with a model where S. flexneri uses IpaH9.8 to induce
autophagy resulting in degradation of host proteins to produce free amino acids and
down-regulate the activity of immune complexes. | en_US |
dc.language.iso | en | en_US |
dc.subject | bacteria | en_US |
dc.subject | microbiology | en_US |
dc.subject | shigella | en_US |
dc.subject | autophagy | en_US |
dc.subject | T3SS | en_US |
dc.subject | effectors | en_US |
dc.title | Shigella Effector IpaH9.8 Interacts with Autophagy Transcription Factor ZKSCAN3 and Increases Autophagy During Infection | en_US |
dc.type | Thesis | |
dc.date.defence | 2014-12-10 | |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Karen Bedard | en_US |
dc.contributor.graduate-coordinator | Brent Johnston | en_US |
dc.contributor.thesis-reader | Roy Duncan | en_US |
dc.contributor.thesis-reader | Jim Fawcett | en_US |
dc.contributor.thesis-supervisor | John Rohde | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |