dc.contributor.author | Soutar, David | |
dc.date.accessioned | 2011-12-02T16:48:03Z | |
dc.date.available | 2011-12-02T16:48:03Z | |
dc.date.issued | 2011-12-02 | |
dc.identifier.uri | http://hdl.handle.net/10222/14329 | |
dc.description.abstract | Piperine, the major alkaloid derived from black pepper corns, has played an important role in traditional medicine worldwide. Current research has demonstrated piperine to have several anti-inflammatory properties, however, little is known concerning the effect of piperine on B cells. Spleen-derived murine B cells were cultured in the presence or absence of piperine during T-dependent or T-independent activation. Piperine reversibly inhibited B cell proliferation in a dose-dependent manner. This was due to a G0/1-phase cell cycle arrest, and was associated with a reduction in phospho-ERK, phospho-AKT, and Cyclin D1, D2, and D3. Piperine also inhibited antibody and cytokine production. Furthermore, piperine treatment diminished B cell-mediated antigen presentation determined by measuring OT-II transgenic T cell proliferation in response to OVA, which was attributed to the decreased MHC-II ad co-stimulatory molecule expression observed. This in vitro study shows that piperine has potent immuno-suppressive effects on B cell activation and effector function. | en_US |
dc.language.iso | en | en_US |
dc.subject | B cell, lymphocyte, piperine, phytochemical, alkaloid, TRPV1 | en_US |
dc.title | Piperine Modulates B cell Activation and Function | en_US |
dc.date.defence | 2011-09-13 | |
dc.contributor.department | Department of Pathology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Jonathan Blay | en_US |
dc.contributor.graduate-coordinator | Dr. Wenda Greer | en_US |
dc.contributor.thesis-reader | Dr. David Hoskin | en_US |
dc.contributor.thesis-reader | Dr. Brent Johnston | en_US |
dc.contributor.thesis-supervisor | Dr. Robert Liwski, Dr. Kenneth West | en_US |
dc.contributor.ethics-approval | Received | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |