dc.contributor.author | Kenward, Calem | |
dc.date.accessioned | 2020-08-07T17:46:59Z | |
dc.date.available | 2020-08-07T17:46:59Z | |
dc.date.issued | 2020-08-07T17:46:59Z | |
dc.identifier.uri | http://hdl.handle.net/10222/79616 | |
dc.description.abstract | Hydrophobins are low molecular weight self-assembling proteins secreted by fungi and are active at hydrophobic-hydrophilic interfaces. Hydrophobins may undergo structural rearrangement and oligomerize to form rodlets, which are an insoluble functional amyloid. To better understand which sequence characteristics determine hydrophobin properties, I have characterized the structure and properties of class IB hydrophobins from various fungi: Serpula lacrymans (SL1), Wallemia ichthyophaga (WI1), and Phanerochaete carnosa (PC1). I determined the high-resolution structure of each hydrophobin using NMR spectroscopy. This revealed that these hydrophobins all share structural features despite their dissimilar sequences. The core conserved feature is a four strand anti-parallel β-sheet that is connected by three loop sequences (L1-L3). In all hydrophobins the β-sheet folds upon itself to form a β-barrel structure. Spectroscopic amyloid formation assays indicate that each hydrophobin has differing propensities to form rodlets. Overall, this work establishes a correlation between the sequence, structure, and self-assembly properties of hydrophobins. | en_US |
dc.language.iso | en | en_US |
dc.subject | Amyloid | en_US |
dc.subject | Hydrophobin | en_US |
dc.subject | Fungal Protein | en_US |
dc.subject | Structural Biology | en_US |
dc.subject | NMR | en_US |
dc.title | Characterization of Structural and Functional Properties of Class IB Hydrophobins | en_US |
dc.type | Thesis | en_US |
dc.date.defence | 2019-03-27 | |
dc.contributor.department | Department of Biochemistry & Molecular Biology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Jan Rainey | en_US |
dc.contributor.thesis-reader | Dr. Andrew Roger | en_US |
dc.contributor.thesis-reader | Dr. Jan Rainey | en_US |
dc.contributor.thesis-reader | Dr. Kathryn Vanya Ewart | en_US |
dc.contributor.thesis-supervisor | Dr. David Langelaan | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |