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Characterization of Structural and Functional Properties of Class IB Hydrophobins

dc.contributor.authorKenward, Calem
dc.contributor.copyright-releaseNot Applicableen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentDepartment of Biochemistry & Molecular Biologyen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Jan Raineyen_US
dc.contributor.thesis-readerDr. Andrew Rogeren_US
dc.contributor.thesis-readerDr. Jan Raineyen_US
dc.contributor.thesis-readerDr. Kathryn Vanya Ewarten_US
dc.contributor.thesis-supervisorDr. David Langelaanen_US
dc.date.accessioned2020-08-07T17:46:59Z
dc.date.available2020-08-07T17:46:59Z
dc.date.defence2019-03-27
dc.date.issued2020-08-07T17:46:59Z
dc.description.abstractHydrophobins are low molecular weight self-assembling proteins secreted by fungi and are active at hydrophobic-hydrophilic interfaces. Hydrophobins may undergo structural rearrangement and oligomerize to form rodlets, which are an insoluble functional amyloid. To better understand which sequence characteristics determine hydrophobin properties, I have characterized the structure and properties of class IB hydrophobins from various fungi: Serpula lacrymans (SL1), Wallemia ichthyophaga (WI1), and Phanerochaete carnosa (PC1). I determined the high-resolution structure of each hydrophobin using NMR spectroscopy. This revealed that these hydrophobins all share structural features despite their dissimilar sequences. The core conserved feature is a four strand anti-parallel β-sheet that is connected by three loop sequences (L1-L3). In all hydrophobins the β-sheet folds upon itself to form a β-barrel structure. Spectroscopic amyloid formation assays indicate that each hydrophobin has differing propensities to form rodlets. Overall, this work establishes a correlation between the sequence, structure, and self-assembly properties of hydrophobins.en_US
dc.identifier.urihttp://hdl.handle.net/10222/79616
dc.language.isoenen_US
dc.subjectAmyloiden_US
dc.subjectHydrophobinen_US
dc.subjectFungal Proteinen_US
dc.subjectStructural Biologyen_US
dc.subjectNMRen_US
dc.titleCharacterization of Structural and Functional Properties of Class IB Hydrophobinsen_US
dc.typeThesisen_US

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