dc.contributor.author | Jones, Quinton RD | |
dc.date.accessioned | 2011-08-15T17:30:48Z | |
dc.date.available | 2011-08-15T17:30:48Z | |
dc.date.issued | 2011-08-15 | |
dc.identifier.uri | http://hdl.handle.net/10222/14058 | |
dc.description | Insulin was used on a model on H9c2 myotubes to determine the effect of oxygen-glucose deprivation and reoxygenation on the localization and phosphorylation of Hsp27 and p38 MAPK | en_US |
dc.description.abstract | Insulin and cellular stressors both activate p38 MAPK. Insulin protects cardiac tissue in a p38 MAPK-dependent manner. Paradoxically, inhibiting p38 MAPK is also protective. Hsp27 phosphorylation is regulated by p38 MAPK. Insulin was tested in H9c2 cardiomyocytes subjected to media exchange, 6 hours of oxygen-glucose deprivation, and reoxygenation. Insulin suppressed stress-induced phosphorylation of Hsp27 due to media-exchange or oxygen-glucose deprivation. Surprisingly, insulin increased Hsp27 phosphorylation during reoxygenation. Insulin also reduced total p38 MAPK levels. Insulin before oxygen-glucose deprivation prevented both localization of Hsp27 to the nucleus and localization of phospho-p38 MAPK to the cytoplasm. Insulin during oxygen-glucose deprivation caused the localization of phospho-p38 MAPK in the cytoplasm, but did not increase Hsp27 phosphorylation until reoxygenation. In conclusion, insulin may protect before oxygen-glucose deprivation by redirecting phospho-p38 MAPK to the nucleus away from damaging pathways in the cytoplasm and protects during oxygen-glucose deprivation by priming phospho-p38 MAPK to phosphorylate Hsp27. | en_US |
dc.language.iso | en | en_US |
dc.subject | insulin h9c2 cardiomyocytes OGD anoxia ischemia hsp27 p38 mapk | en_US |
dc.title | THE EFFECT OF INSULIN ON STRESS-RESPONSE PATHWAYS IN A CELLULAR MODEL OF RAT CARDIOMYOCYTES | en_US |
dc.date.defence | 2011-08-05 | |
dc.contributor.department | Department of Anatomy & Neurobiolgy with Neuroscience | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Bill Baldridge | en_US |
dc.contributor.graduate-coordinator | Kazue Semba | en_US |
dc.contributor.thesis-reader | William Currie, Kazue Semba, Gary Allen, Bill Baldridge, Eileen Denovan-Wright | en_US |
dc.contributor.thesis-supervisor | William Currie | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |