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ESTABLISHING A PATIENT DERIVED ORGANOTYPIC SLICE CULTURE MODEL OF GLIOBLASTOMA MULTIFORME

dc.contributor.authorRappoldt, Liam
dc.contributor.copyright-releaseNot Applicableen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentDepartment of Medical Neuroscienceen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinerDr. Sidney Croulen_US
dc.contributor.graduate-coordinatorDr. Kazue Sembaen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.thesis-readerDr. James Fawcetten_US
dc.contributor.thesis-readerDr. Sultan Darveshen_US
dc.contributor.thesis-supervisorDr. Adrienne Weeksen_US
dc.contributor.thesis-supervisorDr. Craig McCormicken_US
dc.date.accessioned2021-05-06T13:52:39Z
dc.date.available2021-05-06T13:52:39Z
dc.date.defence2021-04-23
dc.date.issued2021-05-06T13:52:39Z
dc.description.abstractGlioblastoma Multiforme (GBM) is the most common primary malignant brain tumour and has a median survival of 15 months. Despite many years of research in the field, prognosis remains dismal, due in part to the majority of the research being performed using monolayer cell culture, which does not represent the complexity of GBM. To partly address this gap in research models, I have generated and utilized a viable patient derived organotypic slice culture model. I have utilized this model to validate genetic manipulation via lentiviral transduction by successfully delivering a far-red fluorescent marker. I further explored the feasibility of utilizing this model to understand patient derived extracellular vesicles (EVs) for the future use as a GBM biomarker in liquid biopsy. The establishment of this model will hopefully lead to insights in to GBM biology and how EVs further its malignancy.en_US
dc.identifier.urihttp://hdl.handle.net/10222/80492
dc.language.isoenen_US
dc.subjectOrganotypicen_US
dc.subjectGlioblastoma Multiformeen_US
dc.subjectCanceren_US
dc.titleESTABLISHING A PATIENT DERIVED ORGANOTYPIC SLICE CULTURE MODEL OF GLIOBLASTOMA MULTIFORMEen_US

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