Repository logo
 

Regulation of Human Aldehyde Oxidase Expression by Retinoic Acid Receptor in MCF-7 Human Breast Adenocarcinoma Cells

dc.contributor.authorAllen, Erica
dc.contributor.copyright-releaseNo
dc.contributor.degreeMaster of Science
dc.contributor.departmentCollege of Pharmacy
dc.contributor.ethics-approvalNot Applicable
dc.contributor.external-examinern/a
dc.contributor.manuscriptsNo
dc.contributor.thesis-readerKerry Goralski
dc.contributor.thesis-readerRemigius Agu
dc.contributor.thesis-supervisorAik Jiang Lau
dc.date.accessioned2024-12-05T15:33:10Z
dc.date.available2024-12-05T15:33:10Z
dc.date.defence2024-11-07
dc.date.issued2024-12-02
dc.description.abstractAldehyde oxidase (AOX) metabolizes various compounds, but its regulation is not well understood. Retinoic acid (RA) has two main isomers: all-trans RA (ATRA), which binds to retinoic acid receptor (RAR), and 9-cis-RA, which binds to RAR and retinoid X receptor (RXR). Although 9-cis-RA and RXR-selective agonists induce AOX1 in MCF-7 human breast adenocarcinoma cells, the role of RAR remains unclear. MCF-7 cells treated with RAR-selective agonists for 96 h showed increased AOX1 expression, which was concentration-dependent for the clinically relevant drugs ATRA and adapalene, as analyzed by real-time PCR. The pan-RAR antagonist AGN193109 or pretreatment with actinomycin D (RNA synthesis inhibitor) attenuated this induction. Targeted RARα knockdown by siRNA revealed partial regulation by this subtype. ELISA assay showed a disconnect between AOX1 mRNA and protein levels. These findings indicate that in MCF-7 cells, RAR regulates AOX1 mRNA but its role in controlling protein expression appears to involve additional regulatory mechanisms.
dc.identifier.urihttps://hdl.handle.net/10222/84722
dc.language.isoen
dc.subjectAldehyde Oxidase
dc.subjectRetinoic Acid Receptors
dc.subjectEnzyme Regulation
dc.titleRegulation of Human Aldehyde Oxidase Expression by Retinoic Acid Receptor in MCF-7 Human Breast Adenocarcinoma Cells

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
EricaAllen2024.pdf
Size:
2.71 MB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.03 KB
Format:
Item-specific license agreed upon to submission
Description: