Regulation of Human Aldehyde Oxidase Expression by Retinoic Acid Receptor in MCF-7 Human Breast Adenocarcinoma Cells
dc.contributor.author | Allen, Erica | |
dc.contributor.copyright-release | No | |
dc.contributor.degree | Master of Science | |
dc.contributor.department | College of Pharmacy | |
dc.contributor.ethics-approval | Not Applicable | |
dc.contributor.external-examiner | n/a | |
dc.contributor.manuscripts | No | |
dc.contributor.thesis-reader | Kerry Goralski | |
dc.contributor.thesis-reader | Remigius Agu | |
dc.contributor.thesis-supervisor | Aik Jiang Lau | |
dc.date.accessioned | 2024-12-05T15:33:10Z | |
dc.date.available | 2024-12-05T15:33:10Z | |
dc.date.defence | 2024-11-07 | |
dc.date.issued | 2024-12-02 | |
dc.description.abstract | Aldehyde oxidase (AOX) metabolizes various compounds, but its regulation is not well understood. Retinoic acid (RA) has two main isomers: all-trans RA (ATRA), which binds to retinoic acid receptor (RAR), and 9-cis-RA, which binds to RAR and retinoid X receptor (RXR). Although 9-cis-RA and RXR-selective agonists induce AOX1 in MCF-7 human breast adenocarcinoma cells, the role of RAR remains unclear. MCF-7 cells treated with RAR-selective agonists for 96 h showed increased AOX1 expression, which was concentration-dependent for the clinically relevant drugs ATRA and adapalene, as analyzed by real-time PCR. The pan-RAR antagonist AGN193109 or pretreatment with actinomycin D (RNA synthesis inhibitor) attenuated this induction. Targeted RARα knockdown by siRNA revealed partial regulation by this subtype. ELISA assay showed a disconnect between AOX1 mRNA and protein levels. These findings indicate that in MCF-7 cells, RAR regulates AOX1 mRNA but its role in controlling protein expression appears to involve additional regulatory mechanisms. | |
dc.identifier.uri | https://hdl.handle.net/10222/84722 | |
dc.language.iso | en | |
dc.subject | Aldehyde Oxidase | |
dc.subject | Retinoic Acid Receptors | |
dc.subject | Enzyme Regulation | |
dc.title | Regulation of Human Aldehyde Oxidase Expression by Retinoic Acid Receptor in MCF-7 Human Breast Adenocarcinoma Cells |