Genetic analysis in Haloferax volcanii.
Date
1991
Authors
Lam, Wan L.
Journal Title
Journal ISSN
Volume Title
Publisher
Dalhousie University
Abstract
Description
Genetic tools have been developed for studying the molecular biology of the halophilic archaebacterium, Haloferax volcanii. Shuttle vectors and a transformation system facilitate direct cloning of archaebacterial genes, and allow the reintroduction of in vitro-altered DNA into Hf. volcanii. The archaebacterial genes isolated have in turn provided the necessary genetic markers for further development of strategies for genetic mapping and for direct manipulation of the chromosome.
Several of these genetic techniques are applied to describe genes for tryptophan biosynthesis and mevalonic acid biosynthesis, to determine the organization of functionally-related genes, and to map auxotrophic mutations to the Hf. volcanii chromosome.
Thesis (Ph.D.)--Dalhousie University (Canada), 1991.
Several of these genetic techniques are applied to describe genes for tryptophan biosynthesis and mevalonic acid biosynthesis, to determine the organization of functionally-related genes, and to map auxotrophic mutations to the Hf. volcanii chromosome.
Thesis (Ph.D.)--Dalhousie University (Canada), 1991.
Keywords
Biology, Molecular.