The Crystal Structure and Quantum Mechanical Treatment of the Anti-Cancer Agent Flavopiridol (Hydochloride) and the Chromone Alkaloid Rohitukin

Abstract

The characterisation of the solid-state crystal structure of the hydrochloric acid salt of anti-cancer agent Flavopiridol (i.e., (–)-2-(2-chlorophenyl)-5,7-dihydroxy-8-[(3S,4R)-3-hydroxy-1-methyl-4-piperidinyl]-4H-1-benzopyran-4-one) is described. The title compound forms meta-stable X-ray quality crystals by slow evaporation of solutions of the material in aqueous methanol. The crystalline unit cell contains two organic cations, two formal chloride counterions and two molecules of methanol, one of which is replaced in 20% occupancy by a water molecule. The crystal form is of space group P1 with cell parameters a = 7.2014(10) Å, b = 12.0094(9) Å, c = 12.6581(14) Å, α = 89.146(4)°, β = 89.788(6)° and γ = 82.180(4)°. The unit cell volume is 1084.4(2)Å3. The general structural features of individual (gas-phase) molecules of protonated Flavopiridol and the naturally occurring 2-methyl-[4H]-chromen-4-one analogue Rohitukine have been calculated by application of Density Functional Theory (dft) at the b3lyp/6-31g* level of sophistication. These results are compared to the reported solid-state data of these two biologically relevant flavanoids.