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THE STRUCTURE-FUNCTION RELATIONSHIP BETWEEN ASCOPHYLLUM NODOSUM POLYSACCHARIDES AND IN VITRO PREBIOTIC ACTIVITY: AN ASSESSMENT OF THE IMPACT OF EXTRACTION TECHNOLOGIES

dc.contributor.authorOkolie, Chigozie
dc.contributor.copyright-releaseYesen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentFaculty of Agricultureen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Haibo Niuen_US
dc.contributor.manuscriptsYesen_US
dc.contributor.thesis-readerDr. Stephanie Collinsen_US
dc.contributor.thesis-readerDr. Chibuike Udenigween_US
dc.contributor.thesis-supervisorDr. Nancy Pittsen_US
dc.contributor.thesis-supervisorDr. Beth Masonen_US
dc.date.accessioned2018-03-20T18:39:04Z
dc.date.available2018-03-20T18:39:04Z
dc.date.defence2018-02-16
dc.date.issued2018-03-20T18:39:04Z
dc.description.abstractThe impact of four extraction processes (conventional chemical (CCE), microwave-assisted (MAE), ultrasound-assisted (UAE), and enzyme-assisted extraction (EAE)) on the structure-function relationship between polysaccharides from Ascophyllum nodosum and prebiotic functioning was investigated. Fucoidan extracts from the MAE method had significantly (P < 0.05) higher fucose content and sulphate levels, higher uronic acid content, dispersity index, and lower molecular weight, as compared to other extracts. For sodium alginate, all four extracts had similar dispersity index and M/G ratio, and molecular weight range of 65 – 182 kDa; uronic acid content was highest in the MAE extract. Fucoidan from all four methods significantly improved the growth rate of Lactobacillus delbruecki at 0.1% and 0.5% inclusion concentrations, whereas no significant difference was observed for alginate extracts, relative to the un-supplemented strain. Also, fucoidan and alginate supplemented media had no significant impact on the growth rate of Lactobacillus casei as compared to the un-supplemented media.en_US
dc.identifier.urihttp://hdl.handle.net/10222/73788
dc.language.isoenen_US
dc.subjectPrebioticsen_US
dc.subjectExtraction technologiesen_US
dc.subjectFucoidanen_US
dc.subjectAlginateen_US
dc.subjectSeaweeden_US
dc.titleTHE STRUCTURE-FUNCTION RELATIONSHIP BETWEEN ASCOPHYLLUM NODOSUM POLYSACCHARIDES AND IN VITRO PREBIOTIC ACTIVITY: AN ASSESSMENT OF THE IMPACT OF EXTRACTION TECHNOLOGIESen_US

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