ASSESSMENT OF THE POTENTIAL OF MINKERY WASTEWATERS FOR THE PRODUCTION OF DUNALIELLA SALINA AND β-CAROTENE.
dc.contributor.author | MacEachern, Craig | |
dc.contributor.copyright-release | Not Applicable | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.department | Faculty of Agriculture | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Gordon Price | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.thesis-reader | Dr. Qamar Zaman | en_US |
dc.contributor.thesis-reader | Dr. Sophia He | en_US |
dc.contributor.thesis-supervisor | Dr. Haibo Niu | en_US |
dc.date.accessioned | 2018-08-31T18:13:42Z | |
dc.date.available | 2018-08-31T18:13:42Z | |
dc.date.defence | 2018-07-30 | |
dc.date.issued | 2018-08-31T18:13:42Z | |
dc.description | This study aims to assess the viability of mink wastewater as a nutrient source in the production of Dunaliella salina. As a means of cost offsetting, carotenogenesis potential in Dunaliella salina was also explored through exposure to elevated light intensity. Beta-carotene is the primary carotenoid produced in Dunaliella salina and being that it is a pro-vitamin A carotenoid and the fact that vitamin A deficiency is the second most common vitamin deficiency globally there is certainly a market for such a product. Finally this study explored a variety of metabolic pathways in order to assess the suitability for Dunaliella salina to be produced photoautotrophically, heterotrophically and mixotrophically. | en_US |
dc.description.abstract | Dunaliella salina is a species of green microalgae most prominently known for its ability to accumulate large quantities of β-carotene. As part of this study, mink wastewater was incorporated as a nutrient medium as a means of stimulating growth and reducing costs. Mink wastewater, when compared with standard Bold’s Basal Medium saw no significant differences between the nutrient sources in terms of growth (P-value 0.880). This represents a savings of 0.21 CAD per litre of growth medium used at laboratory scale. Following the growth period, a light stress condition of 13 500 lux was introduced using LED bulbs and achieved a maximum β-carotene accumulation of 14.33% of total biomass in lower density cultures. For higher density cultures, maximum β-carotene accumulation would require an increase in illuminance. While Dunaliella salina was able to grow photoautotrophically and mixotrophically it demonstrated an inability to grow heterotrophically in mink wastewater. | en_US |
dc.identifier.uri | http://hdl.handle.net/10222/74198 | |
dc.language.iso | en | en_US |
dc.subject | Microalgae | en_US |
dc.subject | Dunaliella | en_US |
dc.subject | salina | en_US |
dc.subject | carotene | en_US |
dc.subject | carotenoids | en_US |
dc.subject | mink | en_US |
dc.subject | wastewater | en_US |
dc.title | ASSESSMENT OF THE POTENTIAL OF MINKERY WASTEWATERS FOR THE PRODUCTION OF DUNALIELLA SALINA AND β-CAROTENE. | en_US |
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