The GCS1 gene of Saccharomyces cerevisiae.
Date
1994
Authors
Ireland, Linda Sharon.
Journal Title
Journal ISSN
Volume Title
Publisher
Dalhousie University
Abstract
Description
Mutations in the GCS1 gene of Saccharomyces cerevisiae produce a novel reentry-mutant phenotype. At a restrictive temperature, reentry-mutant cells are specifically impaired for resumption of proliferation from stationary phase in response to fresh growth medium. In contrast, the ability of actively dividing mutant cells to maintain ongoing proliferation is not impaired at the temperature that is restrictive for stationary-phase mutant cells. Thus, the reentry-mutant phenotype genetically defines requirements for resumption of proliferation from stationary phase that are distinct from those for maintenance of ongoing proliferation. In this thesis, a molecular and genetic characterization of the GCS1 gene has extended our characterization of the reentry-mutant phenotype. The GCS1 gene sequence predicts a 39-kd polypeptide containing a CxxC(x$\sb $)CxxC putative Zn-finger motif. Either deletion of the GCS1 gene or amino-acid substitution of the 2nd cysteine in the Zn-finger motif produces the same reentry-mutant phenotype, suggesting that the Zn-finger domain is important for Gcs1 protein function.
Thesis (Ph.D.)--Dalhousie University (Canada), 1994.
Thesis (Ph.D.)--Dalhousie University (Canada), 1994.
Keywords
Biology, Molecular.