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Investigation of the ACyl Carrier Protein Binding Partners SpoT and AidB

dc.contributor.authorCottle, Julia Laura
dc.contributor.copyright-releaseYesen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentDepartment of Biochemistry & Molecular Biologyen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinerNot Applicableen_US
dc.contributor.graduate-coordinatorDr. Richard Singeren_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.thesis-readerDr. Richard Singeren_US
dc.contributor.thesis-readerDr. Jan K. Raineyen_US
dc.contributor.thesis-supervisorDr. David Byers and Dr. Christopher McMasteren_US
dc.date.accessioned2010-05-03T15:24:00Z
dc.date.available2010-05-03T15:24:00Z
dc.date.defence2010-04-23
dc.date.issued2010-05-03T15:24:00Z
dc.description.abstractAcyl carrier protein plays an essential role in bacterial fatty acid synthesis and has been recognized as an attractive new antibiotic target. In a previous study using TAP tagged ACP, we identified two ACP binding partners not directly involved in lipid metabolism, AidB and SpoT, that showed increased binding to ACP in stationary phase. In the current project, we found that the increase in binding of SpoT, a stringent response protein, to ACP appears to be due to increased affinity. In contrast, the increase in binding of AidB, an adaptive response protein, appears to be due to increased expression of AidB. Transformation of SpoT-TAP strains with pGEX-ACP vectors followed by purification revealed that SpoT interacts with wild type ACP, but not with ACP mutants that neutralized helix II or cannot fold properly. We used a far western approach to confirm the direct interaction between ACP and AidB in vitro. Overall, these results support the hypothesis that interaction of ACP with SpoT is highly specific and may reflect a regulatory role for ACP, while that with AidB might reflect non-specific interactions.en_US
dc.identifier.urihttp://hdl.handle.net/10222/12818
dc.language.isoenen_US
dc.subjectACPen_US
dc.subjectSpoTen_US
dc.subjectAidBen_US
dc.titleInvestigation of the ACyl Carrier Protein Binding Partners SpoT and AidBen_US

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