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Developing a Sustainable Bioprocess for Chitin Extraction from Atlantic Lobster Shell Waste

dc.contributor.authorSteeves, Helena
dc.contributor.copyright-releaseNot Applicableen_US
dc.contributor.degreeMaster of Applied Scienceen_US
dc.contributor.departmentDepartment of Process Engineering and Applied Scienceen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinerDr. Junzeng Zhangen_US
dc.contributor.graduate-coordinatorDr. Suzanne Budgeen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.thesis-readerDr. Amyl Ghanemen_US
dc.contributor.thesis-supervisorDr. Beth Masonen_US
dc.contributor.thesis-supervisorDr. Su-Ling Brooksen_US
dc.date.accessioned2021-01-07T18:37:55Z
dc.date.available2021-01-07T18:37:55Z
dc.date.defence2019-11-14
dc.date.issued2021-01-07T18:37:55Z
dc.description.abstractThe goal of this research was to develop a fermentative chitin extraction method from Atlantic lobster shell waste. Results showed initial inoculum ratio was the most statistically significant variable in chitin extraction efficiency at a 5% shell ratio. Novel pre-treatments (MAE and UAE) were not found to have a significant influence on extraction efficiency under the tested conditions regardless of the presence of lactose in the pre-treated slurry. As determined by degree of decalcification and deproteinization as well as further measurement of fermentation dynamics, the most efficient of the tested formulations was 5% shell: 5% lactose: 5% inoculum (w/v) without a pre-treatment. The fermentation achieved 95.0% decalcification and 72.7% deproteinization when maintained at 37°C for 5 days with constant stirring. This fermentative approach using lactose and lactobacilli inoculum successfully yielded high levels of mineral and protein removal however further studies are required to optimize the process.en_US
dc.identifier.urihttp://hdl.handle.net/10222/80181
dc.language.isoenen_US
dc.subjectChitinen_US
dc.subjectFermentationen_US
dc.subjectLactic Acid Bacteriaen_US
dc.subjectLobster shellsen_US
dc.titleDeveloping a Sustainable Bioprocess for Chitin Extraction from Atlantic Lobster Shell Wasteen_US
dc.typeThesisen_US

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