The complex kaposin locus of Kaposi's sarcoma-associated herpesvirus: defining a new role in the lytic viral replication cycle
dc.contributor.author | MacNeil, Grant | |
dc.contributor.copyright-release | Not Applicable | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.external-examiner | Dr. Roy Duncan | en_US |
dc.contributor.graduate-coordinator | Dr. Brent Johnston | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.thesis-reader | Dr. Jan Rainey | en_US |
dc.contributor.thesis-reader | Dr. David Langelaan | en_US |
dc.contributor.thesis-supervisor | Dr. Jennifer Corcoran | en_US |
dc.contributor.thesis-supervisor | Dr. Craig McCormick | en_US |
dc.date.accessioned | 2019-04-02T12:46:46Z | |
dc.date.available | 2019-04-02T12:46:46Z | |
dc.date.defence | 2019-03-14 | |
dc.date.issued | 2019-04-02T12:46:46Z | |
dc.description.abstract | Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiologic agent of the endothelial cell (EC) cancer, Kaposi’s sarcoma, and two B-cell malignancies. In infected cells, the virus exists in two states; the latent program alters cell morphology and inflammatory gene expression, while the lytic cycle produces new virus and promotes tumorigenesis in a paracrine manner. The kaposin locus, located in the latent gene cluster, generates multiple transcripts and gene products in both viral states; however, its function remains unclear. I developed a library of novel recombinant viruses to mutagenize kaposin coding capacity and investigate protein-specific effects during each viral life cycle. I found that while entry and latent gene expression were unaffected by kaposin mutagenesis, the ability to reprogram ECs may be impaired. Furthermore, I identified defects in lytic viral gene expression and virion production in some kaposin-mutant viruses, leading me to suggest new roles for the kaposins in lytic cycle progression. | en_US |
dc.identifier.uri | http://hdl.handle.net/10222/75411 | |
dc.language.iso | en | en_US |
dc.subject | KSHV | en_US |
dc.subject | herpesvirus | en_US |
dc.subject | Kaposin | en_US |
dc.subject | lytic replication | en_US |
dc.title | The complex kaposin locus of Kaposi's sarcoma-associated herpesvirus: defining a new role in the lytic viral replication cycle | en_US |
dc.type | Thesis | en_US |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- MacNeil-Grant-MSc-MICI-March-2019.pdf
- Size:
- 111.08 MB
- Format:
- Adobe Portable Document Format
- Description:
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: