Ovine ILL-thrift in Nova Scotia. 14. Anorexia and digestibility decline in female lambs given 3,7,11-3h3-3-acetoxy-7, 15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one

Abstract

3,7,11-^3H3-3-Acetoxy-7,15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one (I, R=H, R'=Ac) was produced by Fusarium culmorum(CMI14764) grown on a defined medium supplemented with 5-^3H2- mevalonic acid. The metabolite was rigorously purified and the position of the 3-^3H label unequivocally determined by tritylation of the 15-hydroxy group. hydrolysis of the ester and regiospecific oxidation of the 3-hydroxy group thus generated. The metabolite (I, R=H, R'=Ac) given as a single intraruminal dose (5 mg kg-^1) to female lambs resulted in a 44% decline in food intake and a 5%decrease in apparent digestibility of this feed, in the 4 days following administration of the toxin (I, R=H, R'=Ac).