DEVELOPMENT AND EVALUATION OF SULFORAPHANE-RICH BROCCOLI OINTMENT FOR ATOPIC DERMATITIS MANAGEMENT IN BALB/C MICE
| dc.contributor.author | Chaturvedi, Chandrika | |
| dc.contributor.copyright-release | No | |
| dc.contributor.degree | Master of Science | |
| dc.contributor.department | Department of Plant, Food and Environmental Sciences | |
| dc.contributor.ethics-approval | Received | |
| dc.contributor.external-examiner | n/a | |
| dc.contributor.manuscripts | No | |
| dc.contributor.thesis-reader | Dr. Yunfei Jiang | |
| dc.contributor.thesis-reader | Dr. Carman A. Giacomantonio | |
| dc.contributor.thesis-supervisor | Dr. H.P. Vasantha Rupasinghe | |
| dc.date.accessioned | 2026-05-19T13:21:23Z | |
| dc.date.available | 2026-05-19T13:21:23Z | |
| dc.date.defence | 2026-05-08 | |
| dc.date.issued | 2026-05-14 | |
| dc.description | This thesis investigates the therapeutic potential of broccoli-derived extracts for the management of atopic dermatitis. It evaluates their effects on key pathological markers, including dermatitis severity, epidermal thickness, and immunological responses such as IgE and chemokine levels. The findings demonstrate that topical formulations exert predominantly localized anti-inflammatory effects, with limited systemic modulation. Notably, variations in sulforaphane content influence efficacy, highlighting its role as a bioactive compound. Overall, the study supports the development of plant-based topical agents for dermatological and cosmeceutical applications. | |
| dc.description.abstract | This study investigated broccoli (Brassica oleracea) as a sustainable source of bioactive phytochemicals and its potential application in atopic dermatitis (AD). Ultrasound-assisted ethanol extraction (25% ethanol, 41°C, 32 min) yielded optimal total glucosinolate content (36.6 mg sinigrin equivalence/g DW) and sulforaphane content (0.38 mg/g DW), demonstrating strong antioxidant and anti-elastase activity. In the 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mouse model, AD-like symptoms were successfully established, confirmed by elevated serum IgE and dermatitis scores. Topical application of 15% broccoli head (BH) and broccoli sprout (BS) extracts significantly reduced dermatitis scores, epidermal thickness, and serum IgE levels. The 15% BS ointment further decreased serum CCL11 levels, consistent with its approximately 17.5-fold higher sulforaphane content. Serum cytokines showed no significant changes, indicating that therapeutic effects were primarily localized within the skin. These findings support broccoli-derived extract ointments as promising natural topical agents for AD management and functional cosmeceutical development. | |
| dc.identifier.uri | https://hdl.handle.net/10222/86076 | |
| dc.language.iso | en | |
| dc.subject | Broccoli (Brassica oleracea var. italica) | |
| dc.subject | Sulforaphane | |
| dc.subject | Atopic dermatitis | |
| dc.title | DEVELOPMENT AND EVALUATION OF SULFORAPHANE-RICH BROCCOLI OINTMENT FOR ATOPIC DERMATITIS MANAGEMENT IN BALB/C MICE |