Repository logo
 

Shigella Effector IpaH9.8 Modulates a Host Ubiquitin-Like Pathway and Lysosomal Biogenesis

dc.contributor.authorJarche, Lucas
dc.contributor.copyright-releaseNot Applicableen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentDepartment of Microbiology & Immunologyen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinerNikhil Thomasen_US
dc.contributor.graduate-coordinatorBrent Johnstonen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.thesis-readerZhenyu Chengen_US
dc.contributor.thesis-readerMelanie Dobsonen_US
dc.contributor.thesis-supervisorJohn Rohdeen_US
dc.contributor.thesis-supervisorCraig McCormicken_US
dc.date.accessioned2020-01-21T19:17:11Z
dc.date.available2020-01-21T19:17:11Z
dc.date.defence2018-11-20
dc.date.issued2020-01-21T19:17:11Z
dc.description.abstractThe bacterium Shigella causes bacterial dysentery in developing countries and represents a persistent burden to healthcare systems due to an increase in antibiotic-resistant strains and lack of a vaccine strategy. Shigella encodes a type III secretion system to inject effector proteins into the eukaryotic cytosol to hijack and disrupt host processes. One of these effectors is IpaH9.8, a Novel E3 ubiquitin Ligase (NEL) that can target host proteins for post-translational modification with ubiquitin. Cellular targets for IpaH9.8 are unknown despite more than a decade of searching. My results are consistent with a model where IpaH9.8 interacts with a ubiquitin-like protein, UFM1. By co- immunoprecipitation, mass spectrometry, and in vitro UFMylation I have identified Galectin-7, a sugar-binding protein, as an IpaH9.8 target. Additionally, I have characterized another IpaH9.8 interactor, ZKSCAN3, a negative regulator of lysosomal biogenesis, and have provided preliminary evidence that Shigella manipulate host lysosomes through ubiquitination of ZKSCAN3.en_US
dc.identifier.urihttp://hdl.handle.net/10222/77299
dc.language.isoenen_US
dc.subjectShigellaen_US
dc.subjectUFM1en_US
dc.subjectUbiquitinen_US
dc.subjectEffectoren_US
dc.subjectIpaHen_US
dc.titleShigella Effector IpaH9.8 Modulates a Host Ubiquitin-Like Pathway and Lysosomal Biogenesisen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Lucas-Jarche-MSc-MICI-November-2018.pdf
Size:
50.44 MB
Format:
Adobe Portable Document Format
Description:
Lucas Jarche MSc Thesis November 2018

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: