Shigella Effector IpaH9.8 Modulates a Host Ubiquitin-Like Pathway and Lysosomal Biogenesis
dc.contributor.author | Jarche, Lucas | |
dc.contributor.copyright-release | Not Applicable | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.external-examiner | Nikhil Thomas | en_US |
dc.contributor.graduate-coordinator | Brent Johnston | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.thesis-reader | Zhenyu Cheng | en_US |
dc.contributor.thesis-reader | Melanie Dobson | en_US |
dc.contributor.thesis-supervisor | John Rohde | en_US |
dc.contributor.thesis-supervisor | Craig McCormick | en_US |
dc.date.accessioned | 2020-01-21T19:17:11Z | |
dc.date.available | 2020-01-21T19:17:11Z | |
dc.date.defence | 2018-11-20 | |
dc.date.issued | 2020-01-21T19:17:11Z | |
dc.description.abstract | The bacterium Shigella causes bacterial dysentery in developing countries and represents a persistent burden to healthcare systems due to an increase in antibiotic-resistant strains and lack of a vaccine strategy. Shigella encodes a type III secretion system to inject effector proteins into the eukaryotic cytosol to hijack and disrupt host processes. One of these effectors is IpaH9.8, a Novel E3 ubiquitin Ligase (NEL) that can target host proteins for post-translational modification with ubiquitin. Cellular targets for IpaH9.8 are unknown despite more than a decade of searching. My results are consistent with a model where IpaH9.8 interacts with a ubiquitin-like protein, UFM1. By co- immunoprecipitation, mass spectrometry, and in vitro UFMylation I have identified Galectin-7, a sugar-binding protein, as an IpaH9.8 target. Additionally, I have characterized another IpaH9.8 interactor, ZKSCAN3, a negative regulator of lysosomal biogenesis, and have provided preliminary evidence that Shigella manipulate host lysosomes through ubiquitination of ZKSCAN3. | en_US |
dc.identifier.uri | http://hdl.handle.net/10222/77299 | |
dc.language.iso | en | en_US |
dc.subject | Shigella | en_US |
dc.subject | UFM1 | en_US |
dc.subject | Ubiquitin | en_US |
dc.subject | Effector | en_US |
dc.subject | IpaH | en_US |
dc.title | Shigella Effector IpaH9.8 Modulates a Host Ubiquitin-Like Pathway and Lysosomal Biogenesis | en_US |
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