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PROBIOTIC METABOLITES OF OLIGOMERIC PROANTHOCYANIDIN REDUCE NICOTINE-DERIVED NITROSAMINE KETONE ACETATE-INDUCED DNA DAMAGE IN HUMAN LUNG EPITHELIAL AND FETAL HEPATIC CELLS IN VITRO

dc.contributor.authorde Wass Thilakarathna, Wasitha Praveen
dc.contributor.copyright-releaseNoen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentFaculty of Agricultureen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Gordon W. Priceen_US
dc.contributor.manuscriptsNoen_US
dc.contributor.thesis-readerDr. Svetlana Yurgelen_US
dc.contributor.thesis-readerDr. Eric Alberten_US
dc.contributor.thesis-supervisorDr. H.P. Vasantha Rupasingheen_US
dc.date.accessioned2018-08-10T17:42:51Z
dc.date.available2018-08-10T17:42:51Z
dc.date.defence2018-04-26
dc.date.issued2018-08-10T17:42:51Z
dc.descriptionM.Sc. Thesisen_US
dc.description.abstractPotential of proanthocyanidin (PAC) in cancer risk reduction is dependant on the biotransformation process by colonic microbiotia. Seven selected microbial metabolites of proanthocyanidins (MMP) were evaluated for their ability to reduce 4-[(acetoxymethyl) nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc)-induced DNA damage in human lung epithelial (BEAS-2B) and fetal hepatic (WRL-68) cells. Pyrogallol (PG) in BEAS-2B cells and pyrocatechol (PC) in WRL-68 cells reduced the DNA damage as measured by the γ-H2A.X assay. Western blot analysis suggested that PG- and PC-mediated DNA damage reduction is driven by alterations in the DNA damage response (DDR) pathways. Oligomeric apple peel (AP)-PAC and commercial grape seed (GS)-PAC were biotransformed by Lactobacillus rhamnosus and Lactobacillus casei. Phenolic metabolites identified after biotransformation of GS-PAC by L. rhamnosus were PG, PC, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, p-coumaric acid, methyl hippuric acids, 4-hydroxyphenylvaleric acid, and sinapic acid. To confirm the PAC-mediated chemoprevention, an experimental animal model of NNKOAc-induced A/J mice can be recommended.en_US
dc.identifier.urihttp://hdl.handle.net/10222/74101
dc.language.isoenen_US
dc.subjectChemopreventionen_US
dc.subjectproanthocyanidinen_US
dc.subjectprobiotic metabolitesen_US
dc.subjectDNA damage responseen_US
dc.subjectbiotransformationen_US
dc.titlePROBIOTIC METABOLITES OF OLIGOMERIC PROANTHOCYANIDIN REDUCE NICOTINE-DERIVED NITROSAMINE KETONE ACETATE-INDUCED DNA DAMAGE IN HUMAN LUNG EPITHELIAL AND FETAL HEPATIC CELLS IN VITROen_US
dc.typeThesisen_US

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