Development of a Method for the Detection of Aleutian Mink Disease Virus in Water Samples

Abstract

Aleutian mink disease virus (AMDV) causes significant loss to the mink industry in Nova Scotia (NS). Contaminated water is a speculated virus source therefore my objective was to develop a method for detection of AMDV in water samples. Initially, the quality and quantity of AMDV DNA extracted by four commercial kits was evaluated by PCR. Next, viral capture by adsorption-elution filtration was tested using spiked water samples. The 1MDS filter method was superior or comparable to the MgCl2 filter method and outperformed the AlCl3 method. Lastly, 16 watershed samples from Southwest NS and 11 samples from two AMDV-infected ranches were tested. Five ranch samples from the two ranches tested positive. Three samples, containing more than 22 virus copies/µL after concentration were quantifiable. Pre-concentration, these samples contained 16,787 to 33,471 virus copies/mL. In conclusion, although further optimization is required, a method for the detection of AMDV in water samples was developed.