Examination of the cell envelope of a spina-producing marine pseudomonad.
| dc.contributor.author | Hoyle, Brian Douglas. | en_US |
| dc.contributor.degree | Ph.D. | en_US |
| dc.date.accessioned | 2014-10-21T12:35:47Z | |
| dc.date.available | 1987 | |
| dc.date.issued | 1987 | en_US |
| dc.description | Marine pseudomonad D71 (MPD71) (NCMB 2018; McGregor-Shaw et al., 1973) was grown in conditions favourable or unfavourable for the production of appendages termed spinae (McGregor-Shaw et al., 1973). Sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and immunoblot analyses revealed a 42 kilodalton (kD) protein only in the outer membrane (OM) of spined cells. This protein was concluded to be the spina promoter (spinin; Easterbrook and Coombs, 1976). Spined, unspined, and "despined" OMs contained immunodetectable 65 kD and 21-24 kD ("low MWr") proteins using hyperimmune antispinin antiserum absorbed of cross-reactive antibodies using Escherichia coli K12 and Pseudomonas aeruginosa PAO envelope. Further absorption with unspined cells eliminated the 65 kD species from immunoblots. The relatedness of the 65 kD and "low MWr" species to spinin was not resolved. | en_US |
| dc.description | Envelope perturbants affected spination. However, the action of procaine and phenethyl alcohol was different than that of carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The inhibition of spination by CCCP was coincident with the immunological detection of a protein slightly higher in MWr (2 kD) than spinin. | en_US |
| dc.description | Finally, SDS-PAGE comparison of the proteinase K insoluble material from unspined and spined samples indicated a rough type of structure for the MPD71 LPS. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1987. | en_US |
| dc.identifier.other | AAINN65123 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55245 | |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | en_US | |
| dc.subject | Biology, Microbiology. | en_US |
| dc.title | Examination of the cell envelope of a spina-producing marine pseudomonad. | en_US |
| dc.type | text | en_US |
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