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MICRORNA SPECIES REGULATE IMMUNE PROCESSES: A FOCUS ON NK CELLS IN CHRONIC ARTHRITIS

dc.contributor.authorHargreaves, Breanna Kelly Virginia
dc.contributor.copyright-releaseNot Applicableen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.departmentDepartment of Microbiology & Immunologyen_US
dc.contributor.ethics-approvalReceiveden_US
dc.contributor.external-examinerDr. Shashi Gujaren_US
dc.contributor.graduate-coordinatorDr. Brent Johnstonen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.thesis-readerDr. Thomas Issekutzen_US
dc.contributor.thesis-readerDr. Craig McCormicken_US
dc.contributor.thesis-supervisorDr. Beata Derfalvien_US
dc.contributor.thesis-supervisorDr. Jeanette Boudreauen_US
dc.date.accessioned2021-01-07T18:30:46Z
dc.date.available2021-01-07T18:30:46Z
dc.date.defence2019-06-20
dc.date.issued2021-01-07T18:30:46Z
dc.description.abstractRheumatoid arthritis (RA) is a common chronic autoimmune/inflammatory disease involving innate/adaptive lymphocytes and soluble mediators. Natural killer (NK) cells are innate lymphocytes that direct immunity through cytotoxicity and cytokine production. The contributions of NK cells are ill-defined in RA, although their function(s) may be regulated by microRNAs (miRNAs). MiRNAs are non-coding RNAs that inhibit mRNA. To investigate the miRNA-NK cell interplay, we optimized highly efficient transfection techniques in serum-free media for enhancing or interrupting NK cell miRNA signaling. We targeted miRNAs that are expressed differently in patients with RA compared to healthy individuals. Our findings suggest that miR-146a-5p and miR-155-5p, two miRNAs upregulated in RA, reduce NKG2C expression, a hallmark of adaptive NK cells. We expect chronic overexpression of these miRNAs inhibits adaptive NK cells and their ability to eliminate auto-reactive B/T cells through antibody dependent cellular cytotoxicity (ADCC), thereby reducing treatment efficacy for therapies that rely on effective ADCC.en_US
dc.identifier.urihttp://hdl.handle.net/10222/80179
dc.language.isoenen_US
dc.subjectNatural Killer Cellen_US
dc.subjectMicroRNAen_US
dc.subjectRheumatoid Arthritisen_US
dc.subjectTransfectionen_US
dc.titleMICRORNA SPECIES REGULATE IMMUNE PROCESSES: A FOCUS ON NK CELLS IN CHRONIC ARTHRITISen_US
dc.typeThesisen_US

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