dc.contributor.author | Sivapalan, Kajanan | |
dc.date.accessioned | 2021-08-31T17:56:26Z | |
dc.date.available | 2021-08-31T17:56:26Z | |
dc.date.issued | 2021-08-31T17:56:26Z | |
dc.identifier.uri | http://hdl.handle.net/10222/80783 | |
dc.description | Stem and progenitor cell-based transplantation therapies are emerging as supplemental strategies for myocardial repair. However, life-threatening ventricular arrhythmias are frequently reported as adverse side effects of cell transplantation in both preclinical and clinical studies possibly due to the formation of ectopic pacemaking cells. Therefore, it is important to understand the mechanisms regulating cardiac conduction system (CCS) cell development. My master’s research will focus on the identification of activators and inhibitors that play a role in the VCS cell development through the analysis of Purkinje cell (PC) generation in embryonic mouse ventricular cell cultures. | en_US |
dc.description.abstract | Myocardial regenerative treatments with cardiac progenitor cells (CPCs) have been emerging as viable treatment options for myocardial injury. CPCs have the potential to differentiate into working cardiomyocytes and conduction system cardiomyocytes, however mechanisms of differentiation have yet to be studied. In this study the effect of b -adrenergic stimulation, with Isoproterenol (ISO), and inhibition, with metoprolol (METO), were analyzed on ventricular conduction system (VCS) generation from embryonic day 11.5 mice ventricles (containing populations of CPCs). The effects of supplementation of a reducing agent, ascorbic acid (AA), were also studied on enhancement of ISO actions on VCS gene expression. This study showed that both ISO-alone and in combination with AA was able to enhance VCS marker gene expression, through an increase in cAMP. Furthermore, METO was able to negate the ISO-induced increases in VCS gene expression and cAMP levels. This study indicates a role for b-adrenergic receptor signaling in VCS cell development. | en_US |
dc.language.iso | en | en_US |
dc.subject | Conduction System | en_US |
dc.subject | Beta- Adrenergic Receptor Signalling | en_US |
dc.subject | Isoproterenol | en_US |
dc.title | The Effects of Beta-Adrenergic Receptor Signalling on Ventricular Conduction System Cell Development | en_US |
dc.type | Thesis | en_US |
dc.date.defence | 2021-08-12 | |
dc.contributor.department | Department of Pharmacology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Denis Dupre | en_US |
dc.contributor.thesis-reader | Dr. Susan Howlett | en_US |
dc.contributor.thesis-reader | Dr. Ketul Chaudhary | en_US |
dc.contributor.thesis-supervisor | Dr. Kishore Pasumarthi | en_US |
dc.contributor.ethics-approval | Received | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |