dc.contributor.author | Kosik, Melissa | |
dc.date.accessioned | 2021-05-05T13:55:05Z | |
dc.date.available | 2021-05-05T13:55:05Z | |
dc.date.issued | 2021-05-05T13:55:05Z | |
dc.identifier.uri | http://hdl.handle.net/10222/80473 | |
dc.description.abstract | Thalassiosira weissflogii, a marine diatom, was inactivated using two bench-scale treatment methods: ultraviolet radiation (UV-C) and a water bath (50°C). After treatment with either UV-C or heat, and a 24-hour dark incubation, cultures were analyzed using a range of monitoring methods to assess viability and vitality: serial dilution culture most probable number (SDC-MPN), cellular ATP concentration, vital stains, and chlorophyll fluorescence. T. weissflogii viable cell concentration was reduced by 3-log after 6 minutes of heat exposure or 148 mJ/cm2 of UV-C radiation. UV-C treatment is far more energy efficient, using less than 1% of the equivalent input energy required for heat treatment. Viability, as determined through a growth assay, is compared against the other monitoring methods. Vitality testing was demonstrated to produce false positive results, especially for the UV-C treated cells. | en_US |
dc.language.iso | en | en_US |
dc.subject | Ultraviolet treatment | en_US |
dc.subject | Phytoplankton | en_US |
dc.subject | Ballast water treatment | en_US |
dc.title | A COMPARISON OF VIABILITY AND VITALITY TESTING METHODS AFTER BENCH SCALE UV- AND HEAT-TREATMENT OF THE DIATOM THALASSIOSIRA WEISSFLOGII | en_US |
dc.type | Thesis | en_US |
dc.date.defence | 2019-08-07 | |
dc.contributor.department | Department of Civil Engineering | en_US |
dc.contributor.degree | Master of Applied Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Hany El Naggar | en_US |
dc.contributor.thesis-reader | Dr. Margaret Walsh | en_US |
dc.contributor.thesis-reader | Dr. Hugh MacIntyre | en_US |
dc.contributor.thesis-supervisor | Dr. Graham Gagnon | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |