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dc.contributor.authorHattie, Luke
dc.date.accessioned2020-09-11T16:11:59Z
dc.date.available2020-09-11T16:11:59Z
dc.identifier.urihttp://hdl.handle.net/10222/79833
dc.description.abstractIncreased mitochondrial cholesterol may induce mitochondrial dysfunction and play a role in the progression of neurodegenerative disease and cancer. The voltage-dependent anion channel of the outer mitochondrial membrane has been proposed to participate in cholesterol-related mitochondrial dysfunction through altered associations with proteins involved in metabolism and apoptosis. Current models for studying the effects of mitochondrial cholesterol are disease-based models, blurring the line between true mitochondrial cholesterol effects and other disease effects. Here we present a novel model of increased mitochondrial cholesterol, MitoSTARD4, in which mitochondrial cholesterol content and import to the inner mitochondrial membrane are increased. Cells with increased mitochondrial cholesterol produce more lactate. I explored the mitochondrial associations of voltage-dependent anion channel interacting proteins in cells with elevated mitochondrial cholesterol; BCL-XL, GRP75, PINK1 and PARKIN all have increased mitochondrial presence. The MitoSTARD4 model supports previous findings in disease models that increased mitochondrial cholesterol content induces alterations to mitochondrial function.en_US
dc.language.isoenen_US
dc.subjectMitochondrial cholesterolen_US
dc.subjectMitochondrial dysfunctionen_US
dc.subjectVDACen_US
dc.subjectNeurodegenerationen_US
dc.subjectCanceren_US
dc.titleA Novel Model of Elevated Mitochondrial Cholesterol and The Consequences for Mitochondrial Functionen_US
dc.typeThesisen_US
dc.date.defence2019-08-14
dc.contributor.departmentDepartment of Biochemistry & Molecular Biologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Jan Raineyen_US
dc.contributor.thesis-readerDr. Neale Ridgwayen_US
dc.contributor.thesis-readerDr. Aarnoud van der Spoelen_US
dc.contributor.thesis-supervisorDr. Barbara Kartenen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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