dc.contributor.author | Veinot, Sarah | |
dc.date.accessioned | 2019-12-16T17:06:27Z | |
dc.date.available | 2019-12-16T17:06:27Z | |
dc.date.issued | 2019-12-16T17:06:27Z | |
dc.identifier.uri | http://hdl.handle.net/10222/76813 | |
dc.description.abstract | Kaposi’s sarcoma (KS) is characterized by infection with Kaposi’s sarcoma associated herpesvirus (KSHV), aberrant inflammation and cell spindling. These phenotypes are recapitulated by expression of the viral gene product Kaposin B (KapB). KapB binds and activates the kinase MK2, which is required for both effects; how KapB activates MK2 is not known. MK2 is modified by SUMOylation and preventing SUMOylation enhanced MK2 activity. Therefore, I hypothesized that KapB disrupts MK2 SUMOylation. I confirmed MK2 SUMOylation and discovered that constitutively active MK2 is hyper-SUMOylated compared to wild type, suggesting that SUMOylation may be phosphorylation directed. However, the co-expression KapB did not alter the
MK2 SUMOylation profile in this assay. I observed that overexpressed MK2 constructs stabilized ARE-mRNAs and disassembled PBs. My work uncovered a regulatory interplay between phosphorylation and SUMOylation which may provide insight into the
regulation of inflammatory responses by MK2 during cellular stress, such as latent KSHV infection and tumourigenesis. | en_US |
dc.language.iso | en | en_US |
dc.subject | KSHV | en_US |
dc.subject | SUMOylation | en_US |
dc.subject | MAPKAPK2 | en_US |
dc.subject | MK2 | en_US |
dc.title | The Role of SUMOylation in the KSHV Kaposin B-MK2 Signaling Axis | en_US |
dc.date.defence | 2019-11-14 | |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Karen Bedard | en_US |
dc.contributor.graduate-coordinator | Dr. Brent Johnston | en_US |
dc.contributor.thesis-reader | Dr. Christopher Richardson | en_US |
dc.contributor.thesis-reader | Dr. Melanie Dobson | en_US |
dc.contributor.thesis-supervisor | Dr. Jennifer Corcoran | en_US |
dc.contributor.thesis-supervisor | Dr. John Rohde | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |