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dc.contributor.authorAnthes, Livia Esther
dc.date.accessioned2018-09-28T13:46:55Z
dc.date.available2018-09-28T13:46:55Z
dc.identifier.urihttp://hdl.handle.net/10222/74257
dc.description.abstractThe anti-diabetic drug metformin has been shown to exhibit broad in vitro and in vivo anti-neoplastic activity in various cancer types, including breast cancer. Despite numerous clinical trials ongoing, no targeted biomarkers currently exist to predict or monitor patient response to metformin treatment. Our lab previously conducted quantitative proteomics on MDA-MB-231 triple-negative breast cancer cells conditioned to physiologically-relevant doses of metformin. In this study, I have aimed to evaluate the genes encoding the top 12 most up-regulated proteins for their potential use as metformin biomarkers. I have found that several genes may modulate metformin sensitivity in MDA-MB-231 cells in a glucose-dependent manner. Most notably, I have identified aldoketo reductase family 1 member C3 (AKR1C3) to be a novel metformin response marker and a modifier of metformin sensitivity in breast cancer via both up- and downregulation. AKR1C3 protein expression may also have some predictive value to estimate metformin response in vitro.en_US
dc.language.isoenen_US
dc.subjectMetforminen_US
dc.subjectBreast canceren_US
dc.subjectBiomarkersen_US
dc.subjectAKR1C3en_US
dc.subjectTriple-negative breast canceren_US
dc.titleCharacterizing metformin response and sensitivity biomarkers in breast canceren_US
dc.date.defence2017-07-07
dc.contributor.departmentDepartment of Pathologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Craig McCormicken_US
dc.contributor.graduate-coordinatorDr. Wenda Greeren_US
dc.contributor.thesis-readerDr. Catherine Tooen_US
dc.contributor.thesis-readerDr. Paola Marcatoen_US
dc.contributor.thesis-supervisorDr. Graham Dellaireen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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