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dc.contributor.authorBennett, Leah
dc.date.accessioned2018-08-29T11:56:56Z
dc.date.available2018-08-29T11:56:56Z
dc.identifier.urihttp://hdl.handle.net/10222/74163
dc.description.abstractBreast cancer is the most commonly diagnosed cancer in women, and often becomes multidrug-resistant (MDR). Jadomycins are experimental chemotherapeutics that maintain cytotoxicity in MDR breast cancer cells and investigating the jadomycin uptake mechanism is critical. We hypothesized that solute carrier transporters (SLCO) mediate jadomycin uptake, facilitating jadomycin cytotoxicity in MDR breast cancer cells. The objectives were to determine the expression patterns of 11 SLCOs in seven breast cancer cell lines (using quantitative polymerase chain reaction), and then determine the impact of SLCO knockdown on jadomycin cytotoxicity (using lentivirus transduction and MTT assay). The expression of the SLCOs varied with breast cancer cell type and MDR status. Knockdown cells of the highest expressed SLCOs, SLCO4A1 and SLCO3A1 in MCF7-CON cells did not alter jadomycin S or doxorubicin cytotoxicity. The lack of effect of knocking down individual SLCOs suggests that several SLCO transporters may govern jadomycin uptake allowing for their broad-spectrum activity in MDR breast cancer.en_US
dc.language.isoenen_US
dc.subjectBreast Canceren_US
dc.subjectDrug Transportersen_US
dc.subjectSLCOen_US
dc.subjectJadomycinsen_US
dc.subjectMulti-drug Resistanceen_US
dc.titleTHE EFFECTS OF SOLUTE CARRIER ORGANIC ANION TRANSPORTERS ON JADOMYCIN PHARMACOLOGY IN BREAST CANCERen_US
dc.typeThesisen_US
dc.date.defence2018-08-24
dc.contributor.departmentCollege of Pharmacyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. David Jakemanen_US
dc.contributor.thesis-readerDr. Vasantha Rupasingheen_US
dc.contributor.thesis-readerDr. David Jakemanen_US
dc.contributor.thesis-supervisorDr. Kerry Goralskien_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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