Show simple item record

dc.contributor.authorYang, Jiwon
dc.date.accessioned2017-09-15T15:18:27Z
dc.date.available2017-09-15T15:18:27Z
dc.identifier.urihttp://hdl.handle.net/10222/73313
dc.description.abstractStudies of mitochondrial genomes from diverse eukaryotes provide insights into how mitochondria evolved. Previous research revealed several evolutionarily interesting mitochondrial genomes in the taxon Discoba (Excavata), including the most bacteria-like mitochondrial genomes of Jakobida and extensively fragmented mitochondrial genomes of Euglenozoa. I characterized the mitochondrial genomes of amoeba BB2 and Pharyngomonas kirbyi, which represent deep branches within Heterolobosea, the third main group of Discoba. Using phylogenomic analyses, I showed that BB2 and P. kirbyi are sister taxa at the very base of Heterolobosea. I assembled mitochondrial genomes of BB2 (119 kbp) and P. kirbyi (75 kbp) encoding 45 and 48 putative protein-coding genes, respectively. Interestingly, BB2 mitochondrial genes were extensively fragmented by frame-shifts. Comparative analysis of genomic and transcriptomic data revealed that mitochondrial transcripts of BB2 are heavily edited by mononucleotide insertions to produce functional RNAs. Bioinformatics analyses suggested that this RNA editing is very accurate and efficient, and possibly co-transcriptional.en_US
dc.language.isoenen_US
dc.subjectMitochondrial genomeen_US
dc.subjectRNA editingen_US
dc.subjectHeteroloboseaen_US
dc.titleMITOCHONDRIAL GENOME EVOLUTION IN THE DEEP-BRANCHING HETEROLOBOSEIDS AMOEBA ‘BB2’ AND PHARYNGOMONAS KIRBYIen_US
dc.date.defence2016-07-28
dc.contributor.departmentDepartment of Biochemistry & Molecular Biologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Jan Raineyen_US
dc.contributor.thesis-readerDr. John Archibalden_US
dc.contributor.thesis-readerDr. Claudio Slamovitsen_US
dc.contributor.thesis-readerDr. Michael Grayen_US
dc.contributor.thesis-supervisorDr. Andrew Rogeren_US
dc.contributor.thesis-supervisorDr. Alastair Simpsonen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsYesen_US
dc.contributor.copyright-releaseNot Applicableen_US
 Find Full text

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record