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dc.contributor.authorAmararathna, Dehigaspege Inoka Madumani
dc.date.accessioned2017-03-24T17:13:00Z
dc.date.available2017-03-24T17:13:00Z
dc.identifier.urihttp://hdl.handle.net/10222/72743
dc.description.abstractThe protective effect of polyphenol-rich haskap berry extracts against tobacco-specific nitrosamine ketone (NNK)-induced DNA damage was studied in vitro. Normal lung epithelial BEAS-2B cells were exposed to NNK, and to its precursor, 4-[(acetoxymethyl) nitrosamino]-1-(3-pyridyl)-1-butanone (NNKOAc), to induce DNA damage. Polyphenols present in haskap berries were extracted using ethanol and water, separately. Quercetin-3-O-glucoside (Q3G) and cyanidin-3-O-glucoside (C3G) were used as the reference compounds. BEAS-2B cells were pre-incubated with non-cytotoxic concentrations of haskap extracts, Q3G, and C3G separately, and investigated for their protective effects against NNK- and NNKOAc-induced DNA damage. Pre-incubation of BEAS-2B cells with haskap extracts, Q3G, and C3G, significantly suppressed the NNK- and NNKOAc-induced DNA strand breaks, and intracellular reactive oxygen species generation. The protective effect of haskap extracts could be related to their polyphenol content and high antioxidant capacity. An experimental animal model is proposed to further investigate the chemopreventive potential of haskap polyphenols against NNK-induced lung tumorigenesis.en_US
dc.language.isoenen_US
dc.subjectPolyphenolsen_US
dc.subjectHaskap berryen_US
dc.subjectCanceren_US
dc.subjectDNA damageen_US
dc.titleCHEMOPREVENTIVE EFFECT OF HASKAP BERRY POLYPHENOLS AGAINST TOBACCO SPECIFIC NITROSAMINE-INDUCED DNA DAMAGE IN NORMAL LUNG EPITHELIAL CELLS IN VITROen_US
dc.typeThesisen_US
dc.date.defence2017-02-24
dc.contributor.departmentFaculty of Agricultureen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Paola Marcatoen_US
dc.contributor.graduate-coordinatorDr. Haibo Niuen_US
dc.contributor.thesis-readerN/Aen_US
dc.contributor.thesis-readerDr. David W. Hoskinen_US
dc.contributor.thesis-readerDr. Michael Johnstonen_US
dc.contributor.thesis-supervisorDr. Vasantha Rupasingheen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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