Show simple item record

dc.contributor.authorMacLeod, Sarah
dc.date.accessioned2016-04-29T17:57:56Z
dc.date.available2016-04-29T17:57:56Z
dc.identifier.urihttp://hdl.handle.net/10222/71595
dc.descriptionSubmitted in partial fulfillment of the requirements for the degree of Master of Scienceen_US
dc.description.abstractAtrial natriuretic peptide (ANP) is a hormone with numerous effects in the cardiovascular system, including ion channel physiology. Genetic mapping of a family with hereditary atrial fibrillation (AF) revealed a mutation that produced a mutant ANP (mANP). We used patch-clamping and cAMP assays to investigate the electrophysiological effects of ANP and mANP in mouse atrial myocytes. ANP and mANP had no effects in basal conditions. In the presence of the β-adrenergic receptor agonist isoproterenol (ISO), ANP increased ICa,L density, maximal conductance (Gmax) and hyperpolarized the V1/2 of channel activation (V1/2(act)). ISO and ANP also increased cAMP production. In contrast, ISO+mANP decreased ICa,L density, Gmax and depolarized the V1/2(act) and also decreased cAMP production. ANP’s effects on ICa,L were maintained in NPR-C-/- mice, but absent following NPR-A blockade. mANP effects on ICa,L were lost in NPR-C-/- mice. These opposing effects of ANP and mANP may explain how mANP causes AF.en_US
dc.language.isoenen_US
dc.subjectnatriuretic peptidesen_US
dc.subjectnatriuretic peptide receptorsen_US
dc.subjectaction potentialen_US
dc.subjection channelsen_US
dc.subjectatrial fibrillationen_US
dc.subjectcardiac electrophysiologyen_US
dc.titleEffects of wildtype and mutant forms of atrial natriuretic peptide on L-type Ca2+ current in mouse atrial myocytesen_US
dc.date.defence2014-03-17
dc.contributor.departmentDepartment of Physiology & Biophysicsen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Stacy O'Blenesen_US
dc.contributor.graduate-coordinatorDr. Robert Roseen_US
dc.contributor.thesis-readerDr. Susan Howletten_US
dc.contributor.thesis-readerDr. Xianping Dongen_US
dc.contributor.thesis-supervisorDr. Robert Roseen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
 Find Full text

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record