dc.contributor.author | King, Hunter | |
dc.date.accessioned | 2016-04-26T14:19:48Z | |
dc.date.available | 2016-04-26T14:19:48Z | |
dc.date.issued | 2016-04-26T14:19:48Z | |
dc.identifier.uri | http://hdl.handle.net/10222/71497 | |
dc.description.abstract | Cell testing based on lab-on-chip technology using MEMS devices is a new area of interest for biomedical research, however currently the testing is limited in the sample size which is due to low throughput methods applied for manipulating cell cultures. Here a novel approach is presented to form a contained microfluidic environment on a PolyMUMPs technology based MEMS chip. Within the microfluidic environment, pneumatic and dielectrophoretic pumping technologies are used to manipulate particles for use in the subsequent cell testing applications. These cell testing/squeezing devices would test the mechanical properties of cells for use in biomedical diagnostics. Kraton polymer moulds are placed onto MEMS chips with a placement accuracy of ± 4.0 μm. A working fluid (Sorbitol) is then pumped into the microfluidic channels in the mould by use of a Lucca Technologies GenieTouch™ Syringe Pump. Polystyrene beads are then used to test pneumatic and dielectrophoretic pumping. A controlled pneumatic pumping with velocities up to 30 μm/s was achieved. Using travelling wave dielectrophoresis, bi-directional particle manipulation was achieved with velocities up to 19 μm/s in the wide channels of the mould, however the particle motion was limited to the wide channels only. The 6 μm polystyrene beads were accurately manipulated into the jaws of a mechanical cell squeezing device using pneumatic pumping within the mould channels showing future potential for increasing throughput of lab-on-chip MEMS cell testing devices. | en_US |
dc.language.iso | en | en_US |
dc.subject | MEMS | en_US |
dc.subject | Microfluidics | en_US |
dc.subject | Dielectrophoresis | en_US |
dc.subject | Lab-on-Chip | en_US |
dc.title | Direct Integration of Dielectrophoresis, Pneumatic Pumping, and Reversibly Bonded Polymer Moulds for MEMS Based Lab-on-Chip Applications | en_US |
dc.date.defence | 2016-04-20 | |
dc.contributor.department | Department of Mechanical Engineering | en_US |
dc.contributor.degree | Master of Applied Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Peter Allen | en_US |
dc.contributor.thesis-reader | Dr. Marek Kujath | en_US |
dc.contributor.thesis-reader | Dr. Serguei Iakovlev | en_US |
dc.contributor.thesis-supervisor | Dr. Ted Hubbard | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |