Show simple item record

dc.contributor.authorTanner, Kaitlyn
dc.date.accessioned2016-01-14T18:03:21Z
dc.date.available2016-01-14T18:03:21Z
dc.identifier.urihttp://hdl.handle.net/10222/65292
dc.description.abstractShigella spp. cause severe diarrheal disease known as shigellosis and are a major problem is countries where clean water and sanitation are lacking. Shigella spp. use a Type 3 Secretion System to deliver effector proteins into the cytosol of infected human cells. My thesis focuses on an effector of Shigella flexneri, IpaH9.8, and its role in pathogenesis. IpaH9.8 is a member of a structurally related family of enzymes called the novel E3 ligases (NELs). NEL-domain proteins are E3 ubiquitin ligases that target host proteins to alter their fate. I show that IpaH9.8 ubiquitinates ZKSCAN3, a negative regulator of genes required for autophagy, in a cell free system and induces autophagy in vitro. My results are consistent with a model where S. flexneri uses IpaH9.8 to induce autophagy resulting in degradation of host proteins to produce free amino acids and down-regulate the activity of immune complexes.en_US
dc.language.isoenen_US
dc.subjectbacteriaen_US
dc.subjectmicrobiologyen_US
dc.subjectshigellaen_US
dc.subjectautophagyen_US
dc.subjectT3SSen_US
dc.subjecteffectorsen_US
dc.titleShigella Effector IpaH9.8 Interacts with Autophagy Transcription Factor ZKSCAN3 and Increases Autophagy During Infectionen_US
dc.typeThesis
dc.date.defence2014-12-10
dc.contributor.departmentDepartment of Microbiology & Immunologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerKaren Bedarden_US
dc.contributor.graduate-coordinatorBrent Johnstonen_US
dc.contributor.thesis-readerRoy Duncanen_US
dc.contributor.thesis-readerJim Fawcetten_US
dc.contributor.thesis-supervisorJohn Rohdeen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
 Find Full text

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record