STRUCTURED ILLUMINATION MICROSCOPY USING DIGITAL MICROMIRROR DEVICE

Abstract

Structured illumination microscopy (SIM) increases resolution of optical microscopes substantially. However, simulation of this process using mathematical models is still a novel notion. Purpose: To design a program to simulate the process of SIM and study its application on biological samples. Method: Pre-defined images of microscopic samples were blurred and then reconstructed using a simulation designed in MATLAB. Experimental application of SIM and simulated reconstruction of images obtained using a microscope was then performed. Results: Considerably high resolution images were obtained from the simulation, which was also supported by the pixel intensity plots, signal to noise ratio and peak signal to noise ratio analyses. Resolution of images of samples obtained from experimentally performing SIM on a microscope were also similar to their simulated reconstruction. Conclusion: SIM is a desirable option for optimally imaging biological microscopic samples, physically as well as through simulation process.