| dc.contributor.author | McLean, Nancy Lynn. | en_US |
| dc.date.accessioned | 2014-10-21T12:36:39Z | |
| dc.date.available | 1997 | |
| dc.date.issued | 1997 | en_US |
| dc.identifier.other | AAINQ24779 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55520 | |
| dc.description | Red clover genotypes capable of regenerating plantlets in vitro from non-meristem-derived callus are rare. A previous study identified a clone comprised of a group of plantlets regenerated from a hypocotyl-derived callus culture and another group of plants derived from crown divisions of the epicotyl-derived plant. The callus-derived plants of this clone were highly regenerative when reintroduced to callus culture but the epicotyl-derived plants produced nonregenerative callus cultures. The present study had three objectives: (1) to determine the inheritance of the regenerative trait, (2) to examine meiotic pairing configurations in regenerants, and (3) to compare isozyme profiles of regenerative and nonregenerative plants from the clone. Results from F$\rm\sb1,\ F\sb2$ and BC populations are compatible with genetic control of regeneration by two complementary genes. Structural chromosomal abnormalities were not visible in pollen mother cells during meiosis. No differences were found between regenerative and nonregenerative plants for alcohol dehydrogenase, glutamate dehydrogenase or esterase on starch gels. Isoelectric focusing of callus cultures from regenerative and nonregenerative plants revealed that regeneration was accompanied by a reduction in staining intensity and numbers of peroxidase bands compared to nonregenerative cultures. A unique peroxidase band was associated with nonregenerative cultures. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1997. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Genetics. | en_US |
| dc.subject | Agriculture, Plant Culture. | en_US |
| dc.subject | Biology, Plant Physiology. | en_US |
| dc.title | A genetic study of somatic embryogenesis in red clover (Trifolium pratense L.). | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
