| dc.contributor.author | Knee, Rai. | en_US |
| dc.date.accessioned | 2014-10-21T12:35:12Z | |
| dc.date.available | 1997 | |
| dc.date.issued | 1997 | en_US |
| dc.identifier.other | AAINQ24749 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55486 | |
| dc.description | The fibroblast growth factor (FGF-2) gene is bidirectionally transcribed wherein the sense DNA strand yields multiple mRNA species responsible for encoding FGF-2 protein, and the antisense strand generates a single 1.5 kb mRNA. Formation of double-stranded complexes between sense and antisense mRNAs has been suggested as a posttranscriptional mechanism in the regulation of FGF-2 gene expression. The majority of this thesis describes the cloning and characterization of the rat FGF-2 antisense (FGF-AS) mRNA. | en_US |
| dc.description | Northern and RT-PCR analyses of various rat and human tissues reveal that the AS mRNA is expressed in a tissue-specific and developmentally regulated fashion. A neonatal rat liver library was constructed for the isolation of a full-length rat FCF-AS cDNA. Sequence analysis reveals that the rat FGF-AS transcript is a 1.1 kb polyadenylated RNA with a conserved ORF that specifies a novel 35 kDa protein. The rat AS and sense mRNAs contain 425 bp of complementary overlap at their 3$\sp\prime$ regions. Inverse levels of FGF-2 sense and AS mRNA observed in brain, liver, and certain tumor cell lines, are consistent with the possibility of AS regulation of FGF-2 mRNA. | en_US |
| dc.description | The FGF-AS mRNA encodes a 35 kDa protein which is a novel member of the family of MutT/nudix hydrolyases. This diverse family of enzymes includes the antimutator 8-oxo-dGTPases which hydrolyze mutagenic 8-oxo-dCTP and thereby suppress the occurrence of spontaneous oxidative mutagenesis. Sequence-specific antibodies recognized the in vitro translated antisense protein and detected a 35 kDa protein in Western blots of rat and mouse tissues. FGF-AS mRNA expression in rat glioma cells was shown to increase in response to hydrogen peroxide-induced oxidative stress. The abundant expression of FGF-AS in neonatal liver and tissues such as adrenal, kidney and heart, suggests that the functional role of the antisense protein is likely related to the high metabolic function and/or oxidative stress inherent to these tissues. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1997. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Molecular. | en_US |
| dc.title | Molecular cloning and expression of the mammalian basic fibroblast growth factor antisense mRNA. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
