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dc.contributor.authorHe, Runtao.en_US
dc.date.accessioned2014-10-21T12:35:10Z
dc.date.available1997
dc.date.issued1997en_US
dc.identifier.otherAAINQ24745en_US
dc.identifier.urihttp://hdl.handle.net/10222/55482
dc.descriptionDengue virus infection is the leading cause of morbidity and mortality in many tropical countries. The dengue virus envelope protein (E) plays pivotal roles in mediating infection, pathogenesis and host immune response.en_US
dc.descriptionWe investigated mechanisms of antibody neutralization of dengue virus infection and found that neutralization occurs mainly by blocking E protein-mediated virus attachment to host cells. Dengue-immune sera demonstrated a strong correlation between cell attachment-blocking and neutralization activities. These activities were correlated with levels of E-specific antibodies and involved IgG rather than IgM.en_US
dc.descriptionWe found that dengue-specific antibody triggers binding of dengue virus to platelets, which might offer a partial explanation for the thrombocytopenia seen in severe dengue disease. Virus binding was mediated by the E protein and involved a platelet component other than Fc receptor.en_US
dc.descriptionDengue virus E protein was found to form complexes with pre-membrane protein (prM). Previous studies on other flaviviruses suggested that this association is important in virus maturation and release. We found that prM-E association not only forms a heterodimer, but also forms higher order molecular structures. We also presented evidence that the site of prM-E association on E protein is in the carboxy terminal anchoring domain, while cell-binding activity resides in a trypsin-releasable ectodomain of the E protein.en_US
dc.descriptionOne of the barriers of generating recombinant dengue virus vaccine is to find a low-cost and high level expression eukaryotic system. We, for the first time, expressed dengue virus E protein in yeast, which was used in antibody epitope mapping studies and which represents a potentially attractive vaccine candidate against dengue virus.en_US
dc.descriptionThesis (Ph.D.)--Dalhousie University (Canada), 1997.en_US
dc.languageengen_US
dc.publisherDalhousie Universityen_US
dc.publisheren_US
dc.subjectBiology, Microbiology.en_US
dc.titleCharacterization of dengue virus envelope protein.en_US
dc.typetexten_US
dc.contributor.degreePh.D.en_US
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