| dc.contributor.author | Schalkwyk, Leonard Cornelis. | en_US |
| dc.date.accessioned | 2014-10-21T12:34:51Z | |
| dc.date.available | 1990 | |
| dc.date.issued | 1990 | en_US |
| dc.identifier.other | AAINN64488 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55195 | |
| dc.description | Integrated bottom up and top down mapping has produced a nearly complete restriction map of the chromosome of Haloferax volcanii (3.5 $\times$ 10$\sp6$ bp) and complete maps of two plasmids of 4.2 $\times$ 10$\sp5$ bp and 8.9 $\times$ 10$\sp4$ bp, using six restriction enzymes. The map of the chromosome is composed of 131 cosmids grouped into nine contigs (groups of overlapping clones) which have been joined into two map fragments by pulsed field gel analysis. Regions with restriction site frequencies markedly different from that of the chromosome as a whole are located in one third of the genome, which is also very rich in copies of the insertion sequence ISH51 and two other, less well-characterized repetitive elements. Genes which can be located on the map by molecular hybridization are in general located outside of the site-rich region. The genes mapped include the two ribosomal RNA operons and 39 tRNA genes, of which ten had previously been sequenced, and a variety of protein-coding genes which have been cloned from Haloferax volcanii and other halobacteria. The two rRNA operons are opposite in orientation and nearly diametrically opposite each other on the chromosome, which is probably circular. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1990. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Molecular. | en_US |
| dc.subject | Biology, Microbiology. | en_US |
| dc.title | Studies on the structure of the genome of Haloferax volcanii. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
