dc.contributor.author | Ruzic-Gauthier, Michael | |
dc.date.accessioned | 2013-09-17T13:56:07Z | |
dc.date.available | 2013-09-17T13:56:07Z | |
dc.date.issued | 2013-09-17 | |
dc.identifier.uri | http://hdl.handle.net/10222/36323 | |
dc.description.abstract | DNA photocleaving reagents are a unique class of molecules that display the
ability to cleave DNA, causing strand breaks, upon exposure to an irradiation source. In
terms of biological applications, achieving excitation through a two-photon absorption
event provides for unique benefits that can be useful in such applications as
photodynamic therapy and cell viability studies. Thus, this thesis pertains to the study of a
class of photocleaving reagents that have been shown to become excited through a twophoton
process during irradiation with a pulsed femtosecond laser at 775 nm.
N-(Alkoxy)pyridinethiones were selected as possible oxygen-based radical
generators upon irradiation at two-photon wavelengths. Experiments were carried out
with pBR 322 plasmid DNA to determine if these N-(alkoxy)pyridinethiones could cause
strand cleavage and if so how efficient they are in doing so. Several compounds were
found to be effective DNA strand cleavers when irradiated at two-photon wavelengths,
displaying the utility of two-photon excitation in biological studies. Rationale is
suggested for the observed variation in cleaving efficiency based on inherent properties of
the generated radicals.
A second study was done to measure the two-photon cross section of the
compound N-(anthracenoyloxy)pyridinethione. The two-photon cross section was found
by measuring the fraction of substrate remaining after specific periods of femtosecond
laser irradiation at 775 nm, and the two-photon cross section was found to be 0.051 GM. | en_US |
dc.language.iso | en | en_US |
dc.subject | two-photon excitation | en_US |
dc.subject | DNA cleavage | en_US |
dc.title | Cleavage of duplex DNA using two-photon excitation of N-(alkoxy)pyridine thiones | en_US |
dc.date.defence | 2013-07-22 | |
dc.contributor.department | Department of Chemistry | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Mark stradiotto | en_US |
dc.contributor.thesis-reader | Dr. Alison Thompson | en_US |
dc.contributor.thesis-reader | Dr. Frances L. Cozens | en_US |
dc.contributor.thesis-reader | Dr. Robert L. White | en_US |
dc.contributor.thesis-supervisor | Dr. Norman P. Schepp | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Yes | en_US |