Kachuk C, Faulkner M, Liu F, Doucette AA. Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins though Transmembrane Electrophoresis. J Proteome Res. 2016 Aug 5;15(8):2634-42. doi: 10.1021/acs.jproteome.6b00199. Copyright © 2016 American Chemical Society after peer review.
Membrane proteins are underrepresented in proteome analysis platforms because of their hydrophobic character, contributing to decreased solubility. Sodium dodecyl sulfate is a favored denaturant in proteomic workflows, ...