dc.contributor.author | Zhao, Lisha | |
dc.date.accessioned | 2011-12-15T18:01:27Z | |
dc.date.available | 2011-12-15T18:01:27Z | |
dc.date.issued | 2011-12-15 | |
dc.identifier.uri | http://hdl.handle.net/10222/14361 | |
dc.description | This is the electronic copy of partition of pepsinogen in aqueous two phase system method. | en_US |
dc.description.abstract | The purification of pepsinogen from the stomach of red perch using aqueous two phase systems (ATPS) formed by polyethylene glycol (PEG) and salt at 4°C was optimized. Salt type, salt concentration, PEG molecular weight and PEG concentration had significant effects on total volume (TV), volume ratio (VR), enzyme activity (AE), protein content (CP), specific activity (SA), purification fold (PF) and recovery yield (RY). (NH4)2SO4 at 15% w/w concentration was selected as the optimum salt type and concentration. PEG 1500 at 18% w/w concentration was selected as the optimum PEG molecular weight and concentration. 15% (NH4)2SO4-18% PEG 1500, the optimal ATPS, was compared with ammonium sulfate fractionation (ASF). ATPS gave better partition of pepsinogen (SA of 5.40 U/mg, PF of 5.20 and RY of 86.6%) than ASF (SA of 2.55 U/mg, PF of 2.46, RY of 70.4%). | en_US |
dc.language.iso | en | en_US |
dc.subject | pepsinogen | en_US |
dc.subject | pepsin | en_US |
dc.subject | aqueous two phase system | en_US |
dc.subject | purification | en_US |
dc.title | PARTITION OF PEPSINOGEN FROM THE STOMACH OF RED PERCH (SEBASTES MARINUS) BY AQUEOUS TWO PHASE SYSTEMS | en_US |
dc.date.defence | 2011-11-29 | |
dc.contributor.department | Department of Process Engineering and Applied Science | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Su-Ling Brooks | en_US |
dc.contributor.graduate-coordinator | Georges Kipouros | en_US |
dc.contributor.thesis-reader | Su-Ling Brooks | en_US |
dc.contributor.thesis-supervisor | Abdel Ghaly and Suzanne Budge | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |