Browsing Faculty of Graduate Studies Online Theses by Subject "Enzymology"
Now showing items 1-4 of 4
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EXPLORING CATALYSIS IN THE MANDELATE RACEMASE SUBGROUP OF THE ENOLASE SUPERFAMILY: SUBTLE DIFFERENCES IN THE CATALYTIC MACHINERY
(2020-09-11)The mandelate racemase (MR) subgroup of the enolase superfamily of enzymes (ENS) is mechanistically diverse but structurally similar. This work focused on conserved active site residues in subgroup members MR and L-fuconate ... -
The Expression of 19F-Labeled Beta-Phosphoglucomutase and the Evaluation of Its Inhibitors
(2018-02-12)β-Phosphoglucomutase (βPGM) is an isomerase that catalyzes the conversion of β-glucose 1-phosphate to glucose 6-phosphate via a 1,6-bisphosphate intermediate. Incorporation of 5-fluorotryptophan (5FW) into βPGM (ie. ... -
Studies on the Inhibition of Mandelate Racemase by Arylboronic Acids
(2023-08-08)Mandelate racemase (MR) is an enzyme that catalyzes the Mg2+-dependent interconversion of (R)- and (S)-mandelate, and has served as a model enzyme for exploring the limit of the available free energy of transition state ... -
STUDYING THE ROLE OF THE DIVALENT METAL CATION ACTIVATOR OF THE CAMBIALISTIC ENZYME MANDELATE RACEMASE
(2016-09-12)Mandalate racemase (MR) from Pseudomonas putida requires a divalent metal cation, usually Mg2+, to catalyze the interconversion of the enantiomers of mandelate. Typical of a cambialistic enzyme, the active site Mg2+ ...