dc.contributor.author | Black, Michelle A | |
dc.contributor.author | Deurveilher, Samuel | |
dc.contributor.author | Seki, Tatsunori | |
dc.contributor.author | Marsh, Daniel R | |
dc.contributor.author | Rutishauser, Urs | |
dc.contributor.author | Rafuse, Victor F | |
dc.contributor.author | Semba, Kazue | |
dc.date.accessioned | 2016-09-26T18:22:43Z | |
dc.date.available | 2016-09-26T18:22:43Z | |
dc.date.issued | 2009-12-03 | |
dc.identifier.citation | This is the peer reviewed version of the following article: Black MA, Deurveilher S, Seki T, Marsh DR, Rutishauser U, Rafuse VF, Semba K. (2009) Role of polysialylated neural cell adhesion molecule in rapid eye movement sleep regulation in rats. European Journal of Neuroscience, 30 (11), 2029-2235. doi: 10.1111/j.1460-9568.2009.07000.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |
dc.identifier.uri | http://hdl.handle.net/10222/72223 | |
dc.identifier.uri | http://dx.doi.org/10.1111/j.1460-9568.2009.07000.x | |
dc.description.abstract | Recent evidence suggests that synaptic plasticity occurs during homeostatic processes including sleep-wake regulation, although underlying mechanisms are not well understood. Polysialylated neural cell adhesion molecule (PSA NCAM) is a transmembrane protein that has been implicated in various forms of plasticity. To investigate whether PSA NCAM is involved in the neuronal plasticity associated with spontaneous sleep-wake regulation and sleep homeostasis, three studies were conducted using rats. First, we showed that PSA NCAM immunoreactivity is present in close proximity to key neurons in several nuclei of the sleep-wake system, including the tuberomammillary hypothalamic nucleus, dorsal raphe nucleus, and locus coeruleus. Second, using Western blot analysis and densitometric image analysis of immunoreactivity, we found that 6 h of sleep deprivation changed neither the levels nor the general location of PSA NCAM in the sleep-wake system. Finally, we injected endoneuraminidase (endo N) intracerebroventricularly to examine the effects of PSA removal on sleep-wake states and EEG slow waves at both baseline and during recovery from 6 h of sleep deprivation. Endo N-treated rats showed a small but significant decrease in baseline rapid eye movement (REM) sleep selectively in the late light phase, and a facilitated REM sleep rebound after sleep deprivation, compared to saline-injected controls. Non-REM sleep and wake were unaffected by endo N. These results suggest that PSA NCAM is not particularly involved in the regulation of wake or non-REM sleep, but plays a role in the diurnal pattern of REM sleep as well as in some aspects of REM sleep homeostasis. | en_US |
dc.description.sponsorship | Canadian Institutes of Health Research (MOP14451 and MOP 93673.) | en_US |
dc.description.sponsorship | Natural Sciences and Engineering Research Council of Canada (NSERC 217301-03 and 217301-2009) to K.S. NSERC Predoctoral Award (PGSA) to M.A.B.. | |
dc.language.iso | en | en_US |
dc.publisher | Federation of European Neuroscience Societies and Blackwell Publishing Ltd (currently Wiley) | en_US |
dc.relation.ispartof | European Journal of Neuroscience | en_US |
dc.subject | circadian rhythms | en_US |
dc.subject | REM sleep | en_US |
dc.subject | sleep deprivation | en_US |
dc.subject | sleep homeostasis | en_US |
dc.subject | synaptic plasticity | en_US |
dc.title | Role of polysialylated neural cell adhesion molecule in rapid eye movement sleep regulation in rats | en_US |
dc.type | Text | en_US |