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dc.contributor.authorGillies, Kelsie
dc.date.accessioned2013-11-22T18:49:42Z
dc.date.available2013-11-22T18:49:42Z
dc.date.issued2013-11-22
dc.identifier.urihttp://hdl.handle.net/10222/39732
dc.description.abstractSignal termination processes of GPCRs are well established, unlike processes that regulate the assembly and intracellular trafficking of these signaling complexes. Bimolecular fluorescence complementation was used to study GPCR dimer formation in two projects. Firstly, the importance of Rab GTPases on the cell surface expression and signaling of two chemokine receptors expressed on prostate cancer cells was examined. Rab GTPases necessary for CXCR4 and CCR2 cell surface expression and signaling were different from those necessary for the CXCR4/CCR2 heterodimer. Therefore, this project emphasizes the importance of studying heterodimers as unique entities from their constituent receptors. Secondly, interactions between molecular chaperones and two coreceptors necessary for HIV infection – CCR5, a chemokine GPCR, and the main HIV receptor, CD4, a glycoprotein – were investigated. Further emphasizing the unique characteristics of GPCR dimers, this project found that molecular chaperones interact differently with CCR5 homodimers, when compared to CCR5/CD4 heterodimers.en_US
dc.language.isoenen_US
dc.subjectGPCRen_US
dc.subjectCXCR4en_US
dc.subjectCCR2en_US
dc.subjectRab GTPaseen_US
dc.subjectAnterograde traffickingen_US
dc.subjectProstate canceren_US
dc.subjectCD4en_US
dc.subjectCCR5en_US
dc.subjectMolecular chaperoneen_US
dc.subjectHIVen_US
dc.titleAn examination of how Rab GTPases and molecular chaperones influence plasma membrane expression of chemokine receptor dimersen_US
dc.date.defence2013-11-07
dc.contributor.departmentDepartment of Pharmacologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorJana Sawynoken_US
dc.contributor.thesis-readerKaren Bedarden_US
dc.contributor.thesis-readerJim Fawcetten_US
dc.contributor.thesis-supervisorDenis Dupréen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseYesen_US
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