Browsing by Subject "Proteomics"
Now showing items 1-13 of 13
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ADVANCES IN PROTEOMIC WORKFLOW FROM CELL LYSIS TO PROTEIN PURIFICATION FOR HIGH-THROUGHPUT MASS SPECTROMETRY ANALYSIS
(2024-04-14)Proteomics aims to characterize the complete set of proteins in a cell, tissue, or organism at a specific state or time, playing a significant role in various fields, notably molecular medicine for biomarker discovery and ... -
Assessment, Development, and Improvement of SDS Depletion Strategies in Mass Spectrometry Proteomic Workflows
(2016-08-22)In proteomics, sodium dodecyl sulfate negatively affects trypsin digestion, chromatography, and mass spectrometry, thus, its effective depletion is critical to any protein sample workflow. A quantitative assessment of SDS ... -
CHARACTERIZING THE EFFECT OF COLONY STIMULATING FACTORS ON MACROPHAGE METABOLISM AND PHENOTYPE
(2023-08-24)Macrophages are a highly specialized group of immune cells and often referred to as sentinels of the immune system due to their ability to phagocytose cellular debris and pathogens, detect danger signals, and produce ... -
Development of Techniques for Removal of Sodium Dodecyl Sulfate for Enhanced Protein Identification and Characterization
(2011-12-13)Mass spectrometry is a powerful tool employed in proteomics; however, sodium dodecyl sulfate (SDS), a surfactant used for protein solubilization, is known to cause severe interference at concentrations greater than 0.01%. ... -
Electrokinetic Removal of Sodium Dodecyl Sulfate from Proteins for Mass Spectrometry Analysis
(2021-12-21)Transmembrane electrophoresis (TME) is an electrokinetic method designed to remove sodium dodecyl sulfate (SDS) from protein samples prior to mass spectrometry analysis. Proteins in solution are stored behind dialysis ... -
Examining the variables which influence strong cation exchange of peptides, and their implications on the first dimension of separation in liquid chromatography tandem mass spectrometry
(2018-08-31)Orthogonal separations are often employed to improve the capacity of mass spectrometry for proteome analysis. The gold standard of orthogonal peptide-level separation utilises strong cation exchange (SCX) coupled to reversed ... -
Investigations of Parameters Impacting Transmembrane Electrophoresis for Improved SDS Depletion of Proteome Samples for Mass Spectrometry
(2018-07-13)Sodium dodecyl sulfate (SDS) is a favoured reagent for proteomic sample preparation but detrimental to down-stream analysis by liquid chromatography and mass spectrometry (LC-MS). This group previously introduced a novel ... -
Methods to Isolate Proteins from Detergent-containing Solutions for Proteome Analysis
(2014-12-10)The use of the detergent sodium dodecyl sulfate (SDS) to assist in the solubilization of protein samples can be highly beneficial in the proteomics workflow. However, SDS is incompatible with LC-MS. Acetone precipitation ... -
Multi-omics Visualization of Nuclear Architecture in the Early Branching Dinoflagellate, Oxyrrhis marina
(2024-09-23)Dinoflagellates are an abundant clade of aquatic protists with a fascinating evolutionary history that led to enigmatic features within the nucleus. It was believed that dinoflagellates lacked histones altogether, instead ... -
Proteomic and functional studies of the influenza A virus PA-X protein
(2019-04-12)Viral RNA endonuclease activity is required for influenza A virus (IAV) replication. This activity resides in the polymerase acidic (PA) protein, which assembles into viral RNA-dependent RNA polymerase (RdRp) complexes and ... -
System-driven Research: Legitimate Experimental Design for Biological/biomedical Research
(2015-03-30)Exciting new high-throughput methodologies permit innovative experimental designs for current biological and biomedical research. Much of this research is “non-hypothesis-driven”: its experiments are not designed around a ... -
TOWARDS BIOMARKER DISCOVERY IN CONGENITAL URINARY TRACT OBSTRUCTION
(2014-05-30)Proteome analysis techonologies are commonly employed for discovery-based biomarker identification studies. This thesis aims to help bridge the gap between analytical technology development and clinical application by ... -
Towards the Development of a Proteomics Workflow for High-throughput Protein Biomarker Discovery
(2010-06-22)Two popular workflows exist for quantitative proteome analysis: two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), with staining to visualize proteins, and multidimensional solution phase separations of isotopically ...