LeBlanc, Amber2023-08-312023-08-312023-08-30http://hdl.handle.net/10222/82889Plastic pollution and microplastics (plastic pieces <5 mm) are global concerns due to detrimental effects on ecosystems and human health. This study investigated microplastic contamination in commercial Homarus americanus lobster tail muscle tissue from various Lobster Fishing Areas across Nova Scotia, Canada. Innovative extraction and analysis protocols were developed and validated for sample preparation and extraction of microplastics in these tissue samples. Results confirmed the presence of microplastics in all sampled muscle tissue. Average microplastic size was 2.2-3 µm (limit of quantification: 2.2 µm). Average particle concentration was 6.65 ± 5.36 MPs/g (ww) and logarithmically increased as size decreased. Eight plastic polymers were identified, including polyethylene vinyl acetate (PVA), polyester (PES), and polysulfone (PLS). Potential sources include marine, single-use, and automotive sectors. These findings illustrate the translocation of microplastic into edible lobster muscle, highlight the need for further research, and emphasize the importance of addressing plastic pollution in marine ecosystems.enMicroplasticsAmerican lobsterMachine learningPlastic pollutionLobster fishingMarine ecosystemsArtificial intelligenceThesis