Junho(Andy), Oh2024-04-152024-04-152024-04-12http://hdl.handle.net/10222/83894This study investigated the extraction of carotenoids and polyphenols from Daucus carota. L using pH-responsive phase-Switchable Deep Eutectic Solvents (SDESs). After screening several SDESs, DL-menthol: Octanoic acid (1:1 molar ratio, SDES A) and Octanioc acid: Decanoic acid (1:1 molar ratio, SDES B), were selected for extraction studies. Optimization using Response Surface Methodology paired with Box-Behnken design was performed on the extraction yield (Total Carotenoids Content (TCC) and Total Polyphenol Content (TPC)). After the optimization, 1.33 ± 0.05 mg/g of TCC and 1.50 ± 0.07 mg/g were obtained using the hydrophobic SDES A and SDES B, respectively. TPC obtained using the hydrophilic phase of SDES B under optimal conditions (0.39 ± 0.01 mg/g) was significantly higher than with SDES A (0.31 ± 0.01 mg/g). Both hydrophobic and hydrophilic bioactives were more stable in SDES B, compared to SDES A. Similar antioxidant activity was detected in polyphenol extracts obtained with both SDESs.enDaucus carota. LDeep Eutectic SolventspH-responsiveCarotenoidsPolyphenolOptimizationResponse Surface MethodologyAntioxidant activityThesis